Three-Component Lysine/Ornithine Decarboxylation System in Lactobacillus saerimneri 30a
| Autor: | Hein Trip, Juke S. Lolkema, Patrick Lucas, Andrea Romano |
|---|---|
| Přispěvatelé: | Groningen Biomolecular Sciences and Biotechnology, Molecular Microbiology, Molecular Genetics, Centro Ricerca e Innovazione, Fondazione Edmund Mach, Instituto Agrario S. Michele all' Adige, University of Groningen, Œnologie, Institut National de la Recherche Agronomique (INRA)-Université Victor Segalen - Bordeaux 2 |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Ornithine
Amino Acid Transport Systems Decarboxylation PH ORNITHINE-DECARBOXYLASE Lysine Biology Ornithine Decarboxylase LACTIC-ACID BACTERIA BIOGENIC-AMINES Microbiology OENOCOCCUS-OENI Ornithine decarboxylase STAPHYLOCOCCUS-EPIDERMIDIS 2015B 03 medical and health sciences chemistry.chemical_compound Cadaverine [SDV.IDA]Life Sciences [q-bio]/Food engineering Escherichia coli Putrescine OPERON [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering EXCHANGE Molecular Biology Ornithine decarboxylase antizyme 030304 developmental biology 0303 health sciences Lysine decarboxylase 030306 microbiology Articles Lactobacillus Settore AGR/15 - SCIENZE E TECNOLOGIE ALIMENTARI chemistry Biochemistry LYSINE DECARBOXYLASE ESCHERICHIA-COLI |
| Zdroj: | Journal of Bacteriology, 195(6), 1249-1254. AMER SOC MICROBIOLOGY Journal of Bacteriology Journal of Bacteriology, American Society for Microbiology, 2013, 195 (6), pp.1249-1254. ⟨10.1128/JB.02070-12⟩ |
| ISSN: | 0021-9193 1098-5530 |
| Popis: | International audience; Lactic acid bacteria play a pivotal role in many food fermentations and sometimes represent a health threat due to the ability of some strains to produce biogenic amines that accumulate in foods and cause trouble following ingestion. These strains carry specific enzymatic systems catalyzing the uptake of amino acid precursors (e.g., ornithine and lysine), the decarboxylation inside the cell, and the release of the resulting biogenic amines (e.g., putrescine and cadaverine). This study aimed to identify the system involved in production of cadaverine from lysine, which has not been described to date for lactic acid bacteria. Strain Lactobacillus saerimneri 30a (formerly called Lactobacillus sp. 30a) produces both putrescine and cadaverine. The sequencing of its genome showed that the previously described ornithine decarboxylase gene was not associated with the gene encoding an ornithine/putrescine exchanger as in other bacteria. A new hypothetical decarboxylation system was detected in the proximity of the ornithine decarboxylase gene. It consisted of two genes encoding a putative decarboxylase sharing sequence similarities with ornithine decarboxylases and a putative amino acid transporter resembling the ornithine/putrescine exchangers. The two decarboxylases were produced in Escherichia coli, purified, and characterized in vitro, whereas the transporter was heterologously expressed in Lactococcus lactis and functionally characterized in vivo. The overall data led to the conclusion that the two decarboxylases and the transporter form a three-component decarboxylation system, with the new decarboxylase being a specific lysine decarboxylase and the transporter catalyzing both lysine/cadaverine and ornithine/putrescine exchange. To our knowledge, this is an unprecedented observation of a bacterial three-component decarboxylation system. |
| Databáze: | OpenAIRE |
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