Identification of a Wnt-induced protein complex by affinity proteomics using an antibody that recognizes a sub-population of β-catenin
| Autor: | Janine L Coates, Nadia J. Kershaw, Richard J. Simpson, Eugene A. Kapp, Cameron J. Nowell, Bruno Catimel, Meredith J. Layton, Maree C. Faux, Nicole Church, Anthony W Burgess |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Proteomics
Beta-catenin Population Adenomatous Polyposis Coli Protein Biophysics Alpha catenin Spodoptera Cell Fractionation Biochemistry Chromatography Affinity Analytical Chemistry Cell Line Mice Tandem Mass Spectrometry Cell Adhesion Sf9 Cells Animals Humans Phosphorylation Cell adhesion education Molecular Biology Wnt Signaling Pathway beta Catenin education.field_of_study biology Wnt signaling pathway Antibodies Monoclonal Molecular biology Recombinant Proteins Cell biology Catenin biology.protein Protein Multimerization alpha Catenin Chromatography Liquid Protein Binding |
| Zdroj: | Biochimica et biophysica acta. 1824(7) |
| ISSN: | 0006-3002 |
| Popis: | β-catenin is a signaling protein with diverse functions in cell adhesion and Wnt signaling. Although β-catenin has been shown to participate in many protein-protein interactions, it is not clear which combinations of β-catenin-interacting proteins form discrete complexes. We have generated a novel antibody, termed 4B3, which recognizes only a small subset of total cellular β-catenin. Affinity proteomics using 4B3, in combination with subcellular fractionation, has allowed us to define a discrete trimeric complex of β-catenin, α-catenin and the tumor suppressor APC, which forms in the cytoplasm in response to Wnt signaling. Depletion of the limiting component of this complex, APC, implicates the complex in mediating Wnt-induced changes in cell-cell adhesion. APC is also essential for N-terminal phosphorylation of β-catenin within this complex. Each component of β-catenin/APC/α-catenin complex co-exists in other protein complexes, thus use of a selective antibody for affinity proteomics has allowed us to go beyond the generation of a list of potential β-catenin-interacting proteins, and define when and where a specific complex forms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|