Changes in hemoglobin profile reflect autologous blood transfusion misuse in sports
| Autor: | Roberto Reverberi, Alessia Finotti, Enrica Fabbri, Nicoletta Bianchi, Christel Zambon, Nicola Lamberti, Jessica Gasparello, Fabio Manfredini, Roberto Gambari, Lucia Carmela Cosenza, Maurizio Govoni, Ilaria Lampronti, Francesca Dalla Corte |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male medicine.medical_specialty Future studies Adolescent Autologous blood Blood transfusion autologous 030204 cardiovascular system & hematology Gastroenterology NO 03 medical and health sciences Blood Transfusion Autologous Hemoglobins 0302 clinical medicine Internal medicine medicine Internal Medicine Humans Biomarkers Doping in Sports Trained subjects Jurisprudence medicine.diagnostic_test Population mean business.industry Complete blood count Emergency Medicine Hemoglobin business 030215 immunology Sports |
| Popis: | The changes in hemoglobin (Hb) profile following autologous blood transfusion (ABT) for the first time were studied for anti-doping purposes. Twenty-four healthy, trained male subjects (aged 18‒40) were enrolled and randomized into either the transfusion (T) or control (C) groups. Blood samples were taken from the T subjects at baseline, after withdrawal and reinfusion of 450 ml of refrigerated or cryopreserved blood, and from C subjects at the same time points. Hematological variables (Complete blood count, Reticulocytes, Immature Reticulocytes Fraction, Red-cell Distribution Width, OFF-hr score) were measured. The Hb types were analyzed by high-performance liquid chromatography and the Hemoglobin Profile Index (HbPI) arbitrarily calculated. Between-group differences were observed for red blood cells and reticulocytes. Unlike C, the T group, after withdrawal and reinfusion, showed a significant trend analysis for both hematological variables (Hemoglobin concentration, reticulocytes, OFF-hr score) and Hb types (glycated hemoglobin-HbA1c, HbPI). The control charts highlighted samples with abnormal values (> 3-SD above/below the population mean) after reinfusion for hematological variables in one subject versus five subjects for HbA1c and HbPI. A significant ROC-curve analysis (area = 0.649, p = 0.015) identified a HbA1c cut-off value ≤ 2.7% associated to 100% specificity of blood reinfusion (sensitivity 25%). Hemoglobin profile changed in trained subjects after ABT, with abnormal values of HbA1c and HbPI in 42% of subjects after reinfusion. Future studies will confirm the usefulness of these biomarkers in the anti-doping field. |
| Databáze: | OpenAIRE |
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