In Vitro Antidiabetic Effects of Isolated Triterpene Glycoside Fraction from Gymnema sylvestre
| Autor: | K.V. Harish Prashanth, H. K. Manonmani, Rashmi S. Shenoy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Article Subject Pharmacology High-performance liquid chromatography Glibenclamide Sucrase 03 medical and health sciences 0302 clinical medicine Triterpene medicine chemistry.chemical_classification biology Glycoside lcsh:Other systems of medicine lcsh:RZ201-999 biology.organism_classification 030104 developmental biology Complementary and alternative medicine chemistry 030220 oncology & carcinogenesis biology.protein GLUT2 Gymnema sylvestre Maltase Research Article medicine.drug |
| Zdroj: | Evidence-Based Complementary and Alternative Medicine, Vol 2018 (2018) Evidence-based Complementary and Alternative Medicine : eCAM |
| ISSN: | 1741-427X |
| DOI: | 10.1155/2018/7154702 |
| Popis: | A triterpene glycoside (TG) fraction isolated and purified from ethanolic extract of Gymnema sylvestre (EEGS) was investigated for blood glucose control benefit using in vitro methods. The HPLC purified active fraction TG was characterized using FTIR, LC-MS, and NMR. The purified fraction (TG) exhibited effective inhibition of yeast α-glucosidase, sucrase, maltase, and pancreatic α-amylase with IC50 values 3.16 ± 0.05 μg/mL, 74.07 ± 0.51, 5.69 ± 0.02, and 1.17 ± 0.24 μg/mL, respectively, compared to control. TG was characterized to be a mixture of triterpene glycosides: gymnemic acids I, IV, and VII and gymnemagenin. In vitro studies were performed using mouse pancreatic β-cell lines (MIN6). TG did not exhibit any toxic effects on β-cell viability and showed protection against H2O2 induced ROS generation. There was up to 1.34-fold increase in glucose stimulated insulin secretion (p |
| Databáze: | OpenAIRE |
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