Anti-inflammatory properties of fructo-oligosaccharides in a calf lung infection model and in mannheimia haemolytica-infected airway epithelial cells
| Autor: | Cai, Yang, Gilbert, Myrthe S., Gerrits, Walter J.J., Folkerts, Gert, Braber, Saskia, Afd Pharmacology, Pharmacology |
|---|---|
| Přispěvatelé: | Afd Pharmacology, Pharmacology |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Chemokine
Respiratory diseases Pasteurella multocida Animal Nutrition medicine.medical_treatment Anti-Inflammatory Agents Oligosaccharides airway inflammation Article Immune system medicine pneumonia Animals TX341-641 Animal model Lung Mannheimia haemolytica Nutrition A549 cell Nutrition and Dietetics biology medicine.diagnostic_test Nutrition. Foods and food supply respiratory diseases business.industry animal model non-digestible oligosaccharides Interleukin Epithelial Cells Pneumonia respiratory system Non-digestible oligosaccharides Diervoeding Pneumonia of Calves Enzootic respiratory tract diseases Disease Models Animal nutrition Bronchoalveolar lavage Cytokine medicine.anatomical_structure Immunology WIAS biology.protein Cattle business Ex vivo Airway inflammation Food Science |
| Zdroj: | Nutrients Nutrients, 13(10) Nutrients, 13(10), 1. MDPI AG Nutrients 13 (2021) 10 Nutrients, Vol 13, Iss 3514, p 3514 (2021) Volume 13 Issue 10 |
| ISSN: | 2072-6643 |
| Popis: | Emerging antimicrobial-resistant pathogens highlight the importance of developing novel interventions. Here, we investigated the anti-inflammatory properties of Fructo-oligosaccharides (FOS) in calf lung infections and in airway epithelial cells stimulated with pathogens, and/or bacterial components. During a natural exposure, 100 male calves were fed milk replacer with or without FOS for 8 weeks. Then, immune parameters and cytokine/chemokine levels in the bronchoalveolar lavage fluid (BALF) and blood were measured, and clinical scores were investigated. Calf primary bronchial epithelial cells (PBECs) and human airway epithelial cells (A549) were treated with Mannheimia haemolytica, lipopolysaccharides (LPS), and/or flagellin, with or without FOS pretreatment. Thereafter, the cytokine/chemokine levels and epithelial barrier function were examined. Relative to the control (naturally occurring lung infections), FOS-fed calves had greater macrophage numbers in BALF and lower interleukin (IL)-8, IL-6, and IL-1β concentrations in the BALF and blood. However, FOS did not affect the clinical scores. At slaughter, FOS-fed calves had a lower severity of lung lesions compared to the control. Ex vivo, FOS prevented M. haemolytica-induced epithelial barrier dysfunction. Moreover, FOS reduced M. haemolytica- and flagellin-induced (but not LPS-induced) IL-8, TNF-α, and IL-6 release in PBECs and A549 cells. Overall, FOS had anti-inflammatory properties during the natural incidence of lung infections but had no effects on clinical symptoms. |
| Databáze: | OpenAIRE |
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