New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection

Autor: Elane de Araújo Saraiva, Carla Cristina Guimarães de Moraes, Gleiciane Schupp de Sena Mesquita, Valíria Duarte Cerqueira, Gustavo Góes Cavalcante, Flávia de Nazaré Leite Barros, Isis Abel, Alessandra Scofield, Ana Cristina Mendes-Oliveira, Maeli Fernanda Silva Ferreira, Renata Cecília Soares de Lima Macedo, Katarine de Souza Rocha
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Acta Amazonica, Vol 50, Iss 4, Pp 305-308 (2020)
ISSN: 0044-5967
Popis: We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. We examined 25 individuals of four marsupial and seven rodent species for the presence of the 16S rRNA gene of Leptospira in the DNA extracted from 47 liver and kidney tissue samples using PCR. We detected positive samples in 12% (3/25) of the individuals, in kidney fragments of two marsupial species (Didelphis marsupialis and Marmosops pinheiroi) and in a liver fragment of one rodent species (Echimys chrysurus). These are the first records of Leptospira spp. in M. pinheiroi and E. chrysurus and it is the first molecular survey of marsupials and rodents in the Brazilian Amazon.
Databáze: OpenAIRE