Neuroprotective effects of thymoquinone against spinal cord ischemia-reperfusion injury by attenuation of inflammation, oxidative stress, and apoptosis
| Autor: | Ozan Kahveci, Bulent Erdogan, Fatih Alagoz, Yahya Guvenc, Ucler Kisa, Ramazan Kahveci, Nurkan Aksoy, Aysun Gökçe, Berker Cemil, Mustafa F. Sargon, Emre Cemal Gokce |
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| Přispěvatelé: | Kırıkkale Üniversitesi, Zonguldak Bülent Ecevit Üniversitesi |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine thymoquinone Apoptosis medicine.disease_cause Antioxidants Random Allocation chemistry.chemical_compound 0302 clinical medicine Malondialdehyde Benzoquinones rat Spinal cord injury Thymoquinone Motor Neurons Caspase 3 General Medicine Neuroprotective Agents medicine.anatomical_structure Spinal Cord Methylprednisolone Reperfusion Injury Anesthesia neuroprotection medicine.symptom medicine.drug Neuroimmunomodulation Inflammation Nitric Oxide Neuroprotection 03 medical and health sciences medicine Animals Rats Wistar spinal cord ischemia-reperfusion injury Spinal Cord Ischemia Tumor Necrosis Factor-alpha business.industry medicine.disease Spinal cord methylprednisolone spinal cord injury Disease Models Animal Oxidative Stress 030104 developmental biology chemistry inflammation business Reperfusion injury 030217 neurology & neurosurgery Oxidative stress Interleukin-1 |
| Zdroj: | Journal of Neurosurgery: Spine. 24:949-959 |
| ISSN: | 1547-5654 |
| DOI: | 10.3171/2015.10.spine15612 |
| Popis: | OBJECTIVE Ischemia-reperfusion (I/R) injury of the spinal cord following thoracoabdominal aortic surgery remains the most devastating complication, with a life-changing impact on the patient. Thymoquinone (TQ), the main constituent of the volatile oil from Nigella sativa seeds, is reported to possess strong antioxidant, antiinflammatory, and antiapoptotic properties. This study investigated the effects of TQ administration following I/R injury to the spinal cord. METHODS Thirty-two rats were randomly allocated into 4 groups. Group 1 underwent only laparotomy. For Group 2, aortic clip occlusion was introduced to produce I/R injury. Group 3 was given 30 mg/kg of methylprednisolone intraperitoneally immediately after the I/R injury. Group 4 was given 10 mg/kg of TQ intraperitoneally for 7 days before induction of spinal cord I/R injury, and administration was continued until the animal was euthanized. Locomotor function (Basso, Beattie, and Bresnahan scale and inclined plane test) was assessed at 24 hours postischemia. Spinal cord tissue samples were harvested to analyze tissue concentrations of malondialdehyde, nitric oxide, tumor necrosis factor–α, interleukin-1, superoxide dismutase, glutathione-peroxidase, catalase, and caspase-3. In addition, histological and ultrastructural evaluations were performed. RESULTS Thymoquinone treatment improved neurological outcome, which was supported by decreased levels of oxidative products (malondialdehyde and nitric oxide) and proinflammatory cytokines (tumor necrosis factor–α and interleukin-1), increased activities of antioxidant enzymes (superoxide dismutase, glutathione-peroxidase, and catalase), as well as reduction of motor neuron apoptosis. Light microscopy and electron microscopy results also showed preservation of tissue structure in the treatment group. CONCLUSIONS As shown by functional, biochemical, histological, and ultrastructural analysis, TQ exhibits an important protective effect against I/R injury of the spinal cord. |
| Databáze: | OpenAIRE |
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