Distinct responses of human granulosa lutein cells after hCG or LH stimulation in a spheroidal cell culture system
| Autor: | Stefanie Daube, Andrea Walz, Detlef Pietrowski, Christoph Keck, Julia Becker |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
endocrine system
medicine.medical_specialty Cell Culture Techniques Biology Chorionic Gonadotropin Human chorionic gonadotropin Luteal Cells Spheroids Cellular Internal medicine Genetics medicine Humans Granulosa Lutein Cell Cells Cultured Protein Kinase C Dose-Response Relationship Drug urogenital system Cell Biology Luteinizing Hormone Cyclic AMP-Dependent Protein Kinases Enzyme Activation medicine.anatomical_structure Endocrinology Theca Cell culture Female Signal transduction Luteinizing hormone Corpus luteum hormones hormone substitutes and hormone antagonists Developmental Biology Hormone |
| Zdroj: | Molecular Reproduction and Development. 74:1312-1316 |
| ISSN: | 1098-2795 1040-452X |
| DOI: | 10.1002/mrd.20696 |
| Popis: | The growth and development of the corpus luteum (CL) is regulated by gonadotropic hormones. It is formed by granulosa cells (GCs), theca cells, and endothelial cells, and is the primary source of circulating progesterone. During early pregnancy only human chorionic gonadotropin (hCG) but not luteinizing hormone (LH) extends the life span of the CL, although hCG and LH interact with the same receptor and have similar actions on the CL. In this study a recently by our group established spheroidal GC culture assay served as a model of CL development on which we compared the actions of the gonadotropic hormones LH and hCG. To find out which signal pathways take part in the hormonal regulation of GC we stimulated GC-spheroids with modulators of protein kinases A and C dependent signaling cascades and determined their impact on sprout forming activity in GC. Our results indicate that PKA-dependent signaling pathways play a major role in mediating the hormonal-induced signaling cascades leading to sprouting in GC. Furthermore, this study strongly indicates that the different effects of hCG and LH in the maintenance of the CL may be reasoned in different signal transduction pathways triggered by hCG or LH. |
| Databáze: | OpenAIRE |
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