Prior Exercise Improves Age-Dependent Vascular Endothelial Growth Factor Downregulation and Angiogenesis Responses to Hind-Limb Ischemia in Old Rats
| Autor: | Carmela Zincarelli, Luca Golino, Emma Sanzari, Vincenzo Cimini, Walter J. Koch, Dario Leosco, Federico Piscione, Michele Ciccarelli, Giovanna Giuseppina Altobelli, Giuseppe Rengo, Franco Rengo, Gabriella De Lisa, Bruno Trimarco, Francesca Fortunato, Gennaro Galasso, Guido Iaccarino |
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| Přispěvatelé: | Leosco, Dario, Rengo, Giuseppe, Iaccarino, Guido, Sanzari, E, Golino, L, De Lisa, G, Zincarelli, C, Fortunato, F, Ciccarelli, M, Cimini, Vincenzo, Altobelli, Gg, Piscione, F, Galasso, Gennaro, Trimarco, Bruno, Koch, Wj, Rengo, Franco |
| Rok vydání: | 2007 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Aging medicine.medical_specialty Pathology Angiogenesis Blotting Western Wistar Ischemia Down-Regulation Neovascularization Physiologic Hindlimb alpha Subunit Neovascularization chemistry.chemical_compound Downregulation and upregulation Physical Conditioning Animal Internal medicine medicine Limb perfusion Animals Rats Wistar Physiologic Analysis of Variance Blood Flow Velocity Hypoxia-Inducible Factor 1 alpha Subunit Rats Blotting Animal business.industry medicine.disease Physical Conditioning Vascular endothelial growth factor Vascular endothelial growth factor A Endocrinology chemistry Hypoxia-Inducible Factor 1 Geriatrics and Gerontology medicine.symptom business Western |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1758-535X 1079-5006 |
| Popis: | Downregulation of hypoxia-inducible factor 1 (HIF-1) and vascular endothelial growth factor (VEGF) are shown to be involved in age-dependent impairment of angiogenesis. In this study, we explore whether prior exercise is able to affect these molecular patterns favorably and to enhance neoangiogenesis in old Wistar rats with hind-limb ischemia. At day 7 after surgery, HIF-1alpha and VEGF expression increased in the ischemic muscle of trained animals. Exercise increased capillary density and limb perfusion as revealed by histologic, angiographic, and dyed bead techniques. Furthermore, exercise capacity and limb trophism have significantly improved in trained aged rats. In these animals, the reduction of VEGF serum levels has reflected the comprehensive improvement in local ischemia evoked by exercise. In conclusion, prior exercise represents a valid tool to counteract age-related molecular alterations resulting in impaired angiogenesis in response to ischemia. |
| Databáze: | OpenAIRE |
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