The recombination rate is not increased when retroviral RNA is missing an encapsidation sequence
| Autor: | H. M. Temin, Jiayou Zhang |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Dimer
Immunology RNA-dependent RNA polymerase Biology Microbiology Cell Line Transduction (genetics) chemistry.chemical_compound Retrovirus Proviruses Chimeric RNA Virology Animals Recombination Genetic Virus Assembly RNA biology.organism_classification Molecular biology chemistry Insect Science RNA Viral Moloney murine leukemia virus Homologous recombination Gene Deletion Spleen Recombination Research Article |
| Zdroj: | Journal of Virology. 70:2019-2021 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Retroviruses, as a result of the presence of two identical RNA molecules in their virions, recombine at a high rate. When nonhomologous RNA is present in the dimer RNA molecules, nonhomologous recombination occurs, although the rate is only 0.1% of the rate of homologous recombination. We developed a protocol to study transduction of cellular proto-oncogenes in a single cycle of retrovirus replication. The psi sequences is a cis required sequence for packaging viral RNA into viral particles. To test if the rate of nonhomologous recombination increases about 1,000 times when the psi sequence is deleted, as reported by other, we modified vectors we used previously (J. Zhang and H. M. Temin, Science 259:234-238, 1993). Our results indicated that the recombination rate did not undergo the increase of about 1,000 times when the psi sequence of a chimeric RNA was deleted. |
| Databáze: | OpenAIRE |
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