Supercritical Carbon Dioxide Treatment of Porous Silicon Increases Biocompatibility with Cardiomyocytes
| Autor: | Chien-Yu Lin, Kai-Hsi Liu, Hsing-Yu Wang, Mei-Hwa Lee, David Jui-Yang Feng, James L. Thomas, Chen-Yuan Chen, Hung-Yin Lin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Silicon
Materials science Biocompatibility Key genes QH301-705.5 Cell Survival Surface Properties chemistry.chemical_element cardiomyocyte Biocompatible Materials Bioengineering Porous silicon Oxygen Catalysis Article Inorganic Chemistry chemistry.chemical_compound angiogenesis Hydrofluoric acid biocompatibility Tissue engineering Etching (microfabrication) Myocytes Cardiac Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Spectroscopy Supercritical carbon dioxide Spectrum Analysis Organic Chemistry fungi technology industry and agriculture General Medicine surface treatment Carbon Dioxide equipment and supplies Computer Science Applications Chemistry porous silicon chemistry Chemical engineering Porosity |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 19 International Journal of Molecular Sciences, Vol 22, Iss 10709, p 10709 (2021) |
| ISSN: | 1422-0067 |
| Popis: | Porous silicon is of current interest for cardiac tissue engineering applications. While porous silicon is considered to be a biocompatible material, it is important to assess whether post-etching surface treatments can further improve biocompatibility and perhaps modify cellular behavior in desirable ways. In this work, porous silicon was formed by electrochemically etching with hydrofluoric acid, and was then treated with oxygen plasma or supercritical carbon dioxide (scCO2). These processes yielded porous silicon with a thickness of around 4 μm. The different post-etch treatments gave surfaces that differed greatly in hydrophilicity: oxygen plasma-treated porous silicon had a highly hydrophilic surface, while scCO2 gave a more hydrophobic surface. The viabilities of H9c2 cardiomyocytes grown on etched surfaces with and without these two post-etch treatments was examined viability was found to be highest on porous silicon treated with scCO2. Most significantly, the expression of some key genes in the angiogenesis pathway was strongly elevated in cells grown on the scCO2-treated porous silicon, compared to cells grown on the untreated or plasma-treated porous silicon. In addition, the expression of several apoptosis genes were suppressed, relative to the untreated or plasma-treated surfaces. |
| Databáze: | OpenAIRE |
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