Ultra rapid liquid chromatography as second dimension in a comprehensive two-dimensional method for the screening of pharmaceutical samples in stability and stress studies
| Autor: | A.L. Huidobro, Peter J. Schoenmakers, Peter Pruim, Coral Barbas |
|---|---|
| Přispěvatelé: | Analytical Chemistry and Forensic Science (HIMS, FNWI) |
| Rok vydání: | 2008 |
| Předmět: |
Electrospray
Chromatography Chemistry Organic Chemistry Analytical chemistry General Medicine Reversed-phase chromatography Mass spectrometry Biochemistry High-performance liquid chromatography Mass Spectrometry Analytical Chemistry Dimension (vector space) Orthogonality Two-dimensional chromatography Pharmaceutical Preparations Impurity Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A, 1190(1-2), 182-190. Elsevier |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2008.02.114 |
| Popis: | Separation orthogonality has been explored with respect to comprehensive two-dimensional liquid chromatography (2D-LC) for different reversed-phase stationary phases. The outcome of this study points out an SB-CN × BEH-C18 combination, used in the first and the second dimension, respectively, as the most orthogonal chromatographic system for the samples assayed. The present investigation reports the employment of an ultra high pressure liquid chromatography system (UPLC) as the second chromatographic dimension, increasing the sensitivity and the speed, completing the whole chromatographic separation in a reasonable time frame. Finally, an off-line 2D-LC method with diode array detection based on the UPLC has been optimized, allowing the separation and minimizing the run time. SB-CN and BEH-C18 were employed as first and second dimension, respectively, with gradient elution applied in each dimension. Alprazolam degraded tablets were studied as a proof of concept of the utility of this type of setups for impurity profiling of complex samples. |
| Databáze: | OpenAIRE |
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