Autophagy in Paracoccidioides brasiliensis under normal mycelia to yeast transition and under selective nutrient deprivation
| Autor: | Flavia Villaça Morais, Diego Santos Onorio, Claudia B. L. Campos, Giselle Ferreira Ribeiro, Caroline Gonçalves de Góes |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Fungal Structure Paracoccidioides Brasiliensis lcsh:Medicine Yeast and Fungal Models Pathology and Laboratory Medicine Paracoccidioides Gene Expression Regulation Fungal Medicine and Health Sciences lcsh:Science Mycelium Fungal Pathogens Multidisciplinary Cell Death Organic Compounds Paracoccidioidomycosis Monosaccharides Eukaryota Chemistry Experimental Organism Systems Cell Processes Medical Microbiology Physical Sciences Cellular Structures and Organelles Pathogens Research Article Autophagic Cell Death 030106 microbiology Carbohydrates Virulence Saccharomyces cerevisiae Mycology Biology Research and Analysis Methods Microbiology Saccharomyces 03 medical and health sciences Model Organisms Autophagy medicine Vesicles Microbial Pathogens Sirolimus Paracoccidioides brasiliensis Adenine lcsh:R Organic Chemistry Organisms Fungi Chemical Compounds Biology and Life Sciences Nutrients Cell Biology medicine.disease biology.organism_classification Yeast Oxidative Stress Glucose 030104 developmental biology Thermally dimorphic fungus lcsh:Q |
| Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 8, p e0202529 (2018) |
| ISSN: | 1932-6203 |
| Popis: | Paracoccidioides spp. is a thermally dimorphic fungus endemic to Latin America and the etiological agent of paracoccidioidomycosis (PCM), a granulomatous disease acquired through fungal propagule inhalation by its mammalian host. The infection is established after successful mycelia to yeast transition in the host pulmonary alveoli. The challenging environment inside the host exposes the fungus to the need of adaptation in order to circumvent nutritional, thermal, oxidative, immunological and other stresses that can directly affect their survival. Considering that autophagy is a response to abrupt environmental changes and is induced by stress conditions, this study hypothesizes that this process might be crucially involved in the adaptation of Paracoccidioides spp. to the host and, therefore, it is essential for the proper establishment of the disease. By labelling autophagous vesicles with monodansylcadaverine, autophagy was observed as an early event in cells during the normal mycelium to yeast transition, as well as in yeast cells of P. brasiliensis under glucose deprivation, and under either rapamycin or 3-methyladenine (3-MA). Findings in this study demonstrated that autophagy is triggered in P. brasiliensis during the thermal-induced mycelium to yeast transition and by glucose-limited conditions in yeasts, both of which modulated by rapamycin or 3-MA. Certainly, further genetic and in vivo analyses are needed in order to finally address the contribution of autophagy for adaptation. Yet, our data propose that autophagy possibly plays an important role in Paracoccidioides brasiliensis virulence and pathogenicity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|