Hormonal regulation of cholesterol 7alpha-hydroxylase specific activity, mRNA levels, and transcriptional activity in vivo in the rat
| Autor: | William M. Pandak, Kaye S. Redford, Richard T. Stravitz, Phillip B. Hylemon, John Y.L. Chiang, Z R Vlahcevic, Douglas M. Heuman |
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| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
Hypophysectomy Cholesterol medicine.medical_treatment Adrenalectomy Thyroid Thyroidectomy QD415-436 Cell Biology Biology Biochemistry chemistry.chemical_compound Endocrinology medicine.anatomical_structure chemistry Internal medicine Gene expression medicine Glucocorticoid medicine.drug Hormone |
| Zdroj: | Journal of Lipid Research, Vol 38, Iss 12, Pp 2483-2491 (1997) |
| ISSN: | 0022-2275 |
| DOI: | 10.1016/s0022-2275(20)30033-x |
| Popis: | In primary cultures of rat hepatocytes, transcription of the cholesterol 7alpha-hydroxylase gene is induced synergistically by glucocorticoid and thyroid hormones. The objective of the present study was to evaluate the role of endogenous glucocorticoid and thyroid hormones in the maintenance of cholesterol 7alpha-hydroxylase gene expression in vivo. Male Sprague-Dawley rats underwent adrenalectomy (A), thyroidectomy (T), adrenalectomy + thyroidectomy (A + T), hypophysectomy (H), or sham surgery (paired controls). Ten days post surgery, livers were harvested and choles terol 7alpha-hydroxylase specific activity, steady-state mRNA levels, and transcriptional activity were determined. Serum corticosterone levels were |
| Databáze: | OpenAIRE |
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