One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9 System
| Autor: | Asuka Onuma, Wataru Fujii, Kunihiko Naito, Koji Sugiura |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Mouse
Biology medicine.disease_cause Gene Knockout Techniques Conditional gene knockout medicine Animals CRISPR Clustered Regularly Interspaced Short Palindromic Repeats Technology Report CRISPR/Cas9 Heritable Gene Alleles Mice Knockout Genetics Mutation Cas9 Phenotype Mice Inbred C57BL Triple knockout Knockout mouse Female Animal Science and Zoology |
| Zdroj: | The Journal of Reproduction and Development |
| ISSN: | 1348-4400 0916-8818 |
| DOI: | 10.1262/jrd.2013-139 |
| Popis: | Triple-knockout mice generated by the one-step CRISPR/Cas9 system were examined for the effects of multiple gene modifications on each phenotype and individual gene function. Sixty embryos were transferred, and 9 pups were obtained; all 9 pups had mutations on 3 loci, and 7 pups showed mutations in all-alleles. F0 mice showed knockout phenotypes or no protein expression of target genes simultaneously, and these mutations were normally inherited in the next generation. |
| Databáze: | OpenAIRE |
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