CL316243 induces phosphatidylinositol 3,4,5-triphosphate production in rat adipocytes in an adenosine deaminase-, pertussis toxin-, or wortmannin-sensitive manner
| Autor: | Y Ohsaka, Y Nomura |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Agonist Physiology medicine.drug_class Adenosine Deaminase Phosphatidylinositol Phosphates Adrenergic beta-3 Receptor Agonists Dioxoles Pertussis toxin Wortmannin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Adenosine deaminase medicine Adipocytes Animals Phosphatidylinositol chemistry.chemical_classification biology AMP deaminase General Medicine Molecular biology Rats Androstadienes 030104 developmental biology Enzyme chemistry Pertussis Toxin 030220 oncology & carcinogenesis biology.protein |
| Zdroj: | Physiological research. 65(3) |
| ISSN: | 1802-9973 |
| Popis: | The effect of beta(3)-adrenoceptor (beta(3)-AR) agonists on adipocytes treated or not treated with signaling modulators has not been sufficiently elucidated. Using rat epididymal adipocytes (adipocytes) labeled with [(32)P]orthophosphate, we found that treatment with the selective beta(3)-AR agonist CL316243 (CL; 1 microM) induces phosphatidylinositol (PI) 3,4,5-triphosphate (PI[3,4,5]P(3)) production and that this response is inhibited by adenosine deaminase (ADA, an adenosine-degrading enzyme; 2 U/ml), pertussis toxin (PTX, an inactivator of inhibitory guanine-nucleotide-binding protein; 1 microg/ml), or wortmannin (WT, a PI-kinase inhibitor; 3 microM). The results showed that CL induced PI(3,4,5)P(3) production in intact adipocytes and that this production was affected by signaling modulators. Taken together, our findings indicate that CL produces PI(3,4,5)P(3) in an ADA-sensitive, PTX-sensitive, or WT-sensitive manner and will advance understanding of the effect of beta(3)-AR agonists on adipocytes. |
| Databáze: | OpenAIRE |
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