A study on the biological function of heat shock factor 1 proteins in breast cancer
| Autor: | Tao Liu, Ming-qian Cao, Bin Wu, Xiao-lan Wang, Jinling Ba, Dian-long Zhang, Chen Nie |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Oncogene Cell growth Chemistry fungi Articles Cell cycle Hsp70 Andrology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine heat shock factor 1 Oncology Apoptosis 030220 oncology & carcinogenesis Heat shock protein macrophage migration inhibitory factor short hairpin RNA Macrophage migration inhibitory factor heat shock protein 70 heat shock protein 90 HSF1 |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1074 |
| Popis: | The present study aimed to investigate the effect of HSF1 proteins on cell proliferation, apoptosis and invasion of breast cancer. The Michigan Cancer Foundation-7 (MCF-7) HSF1-knocked down stable cell line (experimental group) and control cell line (control group) were obtained using a lentivirus assay, and the effects of HSF1 knockdown on the proliferation, tumor formation, apoptosis and invasion ability were analyzed, respectively. The effects of HSF1 on downstream signals were analyzed using western blotting. Western blotting results showed that lentivirus successfully established a HSF1 knockdown stable cell line of MCF-7. Compared with the control group, the growth rate of MCF-7 cells in the experimental group was significantly decreased (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|