Identification of fish CMPK2 as an interferon stimulated gene against SVCV infection
| Autor: | Bo Chen, Yong-An Zhang, Jian Yao, Yuanan Lu, Chen Li, Xueqin Liu, Fang Wang, Ming Kuang, Gehad Elkady, Wanmeng Liu, Yeda Wang, Jiaoyun Liu |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Aquatic Science 03 medical and health sciences chemistry.chemical_compound Fish Diseases RNA interference In vivo Rhabdoviridae Infections Environmental Chemistry Animals Amino Acid Sequence Zebrafish Phylogeny Messenger RNA biology Kinase Interferon-stimulated gene Gene Expression Profiling Cytidine 04 agricultural and veterinary sciences General Medicine Zebrafish Proteins biology.organism_classification Molecular biology In vitro Immunity Innate 030104 developmental biology chemistry Gene Expression Regulation 040102 fisheries 0401 agriculture forestry and fisheries Interferons Rhabdoviridae Nucleoside-Phosphate Kinase Sequence Alignment |
| Zdroj: | Fishshellfish immunology. 92 |
| ISSN: | 1095-9947 |
| Popis: | Cytidine/uridine monophosphate kinase 2 (CMPK2) is known as a nucleoside monophosphate kinase in mitochondria to maintains intracellular UTP/CTP, and could be induced by immunostimulants LPS and Poly (I:C) in mammals, suggesting its potential antiviral and antibacterial role. In this study, CMPK2 was cloned and characterized in Fathead minnow (FHM) cells. In vivo analysis of tissue distribution revealed that CMPK2 transcript was detected in all the tissues of zebrafish (Danio rerio) examined in this study, particularly abundant in liver, spleen and kidney. In addition, indirect immunofluorescence showed that CMPK2 was localized in the cytoplasm of FHM cells. Expression of CMPK2 mRNA was significantly up-regulated following challenge with Spring viraemia of carp virus (SVCV), poly(I:C), or zebrafish IFN1 and IFN3 both in vitro and in vivo. Furthermore, overexpression and RNA interference of CMPK2 in SVCV-infected FHM cells showed significantly antiviral effect. In summary, this study for the first time shows the presence and distribution of CMPK2 in different tissues of zebrafish, but also demonstrates its antiviral potential against SVCV infection in vivo. These new findings could contribute to explain the molecular mechanism of the CMPK2 mediated antiviral function. |
| Databáze: | OpenAIRE |
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