A mandatory role for STAT4 in IL-12 induction of mouse T cell CCR5
| Autor: | Toshiyuki Hamaoka, Chigusa Nakajima, Ping Gao, Masayuki Iwasaki, Michio Tomura, Hiromi Fujiwara, Yi-Fu Yang, Nobuya Yamaguchi, Takao Mukai |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Cell signaling
Receptors CCR5 medicine.drug_class T cell T-Lymphocytes Immunology Receptors Antigen T-Cell Stimulation Biology Monoclonal antibody Lymphocyte Activation Interferon-gamma Mice immune system diseases medicine Immunology and Allergy Animals Humans RNA Messenger skin and connective tissue diseases STAT4 Cells Cultured Mice Knockout Mice Inbred BALB C T-cell receptor Receptors Interleukin-12 hemic and immune systems Receptors Interleukin STAT4 Transcription Factor Flow Cytometry Molecular biology Interleukin-12 Cell biology DNA-Binding Proteins medicine.anatomical_structure Interleukin 12 Trans-Activators Signal Transduction |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 167(12) |
| ISSN: | 0022-1767 |
| Popis: | IL-12 was recently shown to induce CCR5 on TCR-triggered mouse T cells. Considering that STAT4 is the most critical of IL-12 signaling molecules, this study investigated the role for STAT4 in the induction of CCR5 expression. IL-12R was induced by stimulation with anti-CD3 plus anti-CD28 mAb similarly on T cells from wild-type (WT) and STAT4-deficient (STAT4−/−) mice, but the levels of IL-12R induced on IFN-γ-deficient (IFN-γ−/−) T cells were lower compared with WT T cells. Exposure of TCR-triggered WT T cells to IL-12 induced CCR5 expression. In contrast, TCR-triggered STAT4−/− T cells failed to express CCR5 in response to IL-12. IL-12 stimulation induced detectable albeit reduced levels of CCR5 expression on IFN-γ−/− T cells. Addition of rIFN-γ to cultures of IFN-γ−/− T cells, particularly to cultures during TCR triggering resulted in restoration of CCR5 expression. However, CCR5 expression was not induced in STAT4−/− T cells by supplementation of rIFN-γ. These results indicate that for the induction of CCR5 on T cells, 1) STAT4 plays an indispensable role; 2) such a role is not substituted by simply supplementing rIFN-γ; and 3) IFN-γ amplifies CCR5 induction depending on the presence of STAT4. |
| Databáze: | OpenAIRE |
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