Rapid viability polymerase chain reaction method for detection of Francisella tularensis
| Autor: | Staci R. Kane, Teneile M. Alfaro, Sanjiv Shah |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Polymerase Chain Reaction Microbiology Article law.invention Tularemia 03 medical and health sciences chemistry.chemical_compound law medicine Humans Francisella tularensis Molecular Biology Polymerase chain reaction 030304 developmental biology 0303 health sciences Growth medium biology 030306 microbiology Chemistry Infectious dose medicine.disease biology.organism_classification Bioterrorism Slow growth Brain heart infusion Water Microbiology Biological Monitoring |
| Zdroj: | J Microbiol Methods |
| ISSN: | 0167-7012 |
| DOI: | 10.1016/j.mimet.2019.105738 |
| Popis: | Francisella tularensis, which causes potentially fatal tularemia, has been considered an attractive agent of bioterrorism and biological warfare due to its low infectious dose, reported environmental persistence, and ability to be transmitted to humans via multiple exposure routes. Due to slow growth on even selective culture media, detection of viable F. tularensis from environmental and drinking water samples, usually takes > 3 days. Therefore, a rapid viability polymerase chain reaction (RV-PCR) method was developed to detect and identify viable F. tularensis cells in environmental samples. The method uses a change in PCR response during high throughput (48-well) sample incubation in Brain Heart Infusion/Vitox/Fildes/Histidine growth medium to detect viable F. tularensis presence, which is at least two times faster than the current plate culture-based method. Using the method, 10(1) to 10(2) live F. tularensis cells were detected in simulated complex sample matrices containing chemical and biological interferences. |
| Databáze: | OpenAIRE |
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