Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment

Autor: Danilo M. dos Santos, Sarah R. de Annunzio, Juliana C. Carmello, Ana C. Pavarina, Carla R. Fontana, Daniel S. Correa
Přispěvatelé: Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Universidade Estadual Paulista (UNESP)
Rok vydání: 2022
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 2576-6422
Popis: Made available in DSpace on 2022-04-29T08:38:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-17 Periodontitis is a chronic inflammatory disease that can lead to significant destruction of tooth-supporting tissues, compromising dental function and patient's health. Although the currently employed treatment approaches can limit the advance of the disease, the development of multifunctional and hierarchically structured materials is still in demand for achieving successful tissue regeneration. Here, we combine coaxial electrospinning and 3D printing techniques to prepare bilayered zein-based membranes as a potential dual drug delivery platform for periodontal tissue regeneration. A layer of core-sheath electrospun nanofibers consisting of poly(ethylene oxide) (PEO)/curcumin (Curc)/tetracycline hydrochloride (TH) as the core and zein/poly(ϵ-caprolactone)(PCL)/β-glycerolphosphate (β-GP) as the sheath was deposited over a 3D printed honeycomb PLA/zein/Curc platform in order to render a bilayered structure that can mimic the architecture of periodontal tissue. The physicochemical properties of engineered constructs as well as the release profiles of distinct drugs were mainly controlled by varying the concentration of zein (10, 20, 30%, w/w relative to dry PCL) on the sheath layer of nanofibers, which displayed average diameters ranging from 150 to 400 nm. In vitro experiments demonstrated that the bilayered constructs provided sustained release of distinct drugs over 8 days and exhibited biocompatibility toward human oral keratinocytes (Nok-si) (cell viability >80%) as well as antibacterial activity against distinct bacterial strains including those of the red complex such as Porphyromonas gingivalis and Treponema denticola, which are recognized to elicit aggressive and chronic periodontitis. Our study reveals the potential of zein-based bilayered membranes as a dual drug delivery platform for periodontal tissue regeneration. Nanotechnology National Laboratory for Agriculture (LNNA) Embrapa Instrumentação, São Paulo UNESP São Paulo State University School of Pharmaceutical Sciences Department of Clinical Analysis Rodovia Araraquara Jaú, Km 01-s/n-Campos Ville, São Paulo UNESP São Paulo State University School of Dentistry Department of Dental Materials and Prosthodontics, Rua Humaitá, 1680-Centro, São Paulo UNESP São Paulo State University School of Pharmaceutical Sciences Department of Clinical Analysis Rodovia Araraquara Jaú, Km 01-s/n-Campos Ville, São Paulo UNESP São Paulo State University School of Dentistry Department of Dental Materials and Prosthodontics, Rua Humaitá, 1680-Centro, São Paulo
Databáze: OpenAIRE
Externí odkaz: