Expulsion of demecolcine-induced micronuclei from mouse bone marrow polychromatic erythrocytes
| Autor: | Joseph W. Parton, Michael L. Garriott, Joe E. Beyers, J. T. MacGregor |
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| Rok vydání: | 1991 |
| Předmět: |
Male
Erythrocytes Epidemiology Health Toxicology and Mutagenesis Mice Inbred Strains Biology complex mixtures Mice Clastogen chemistry.chemical_compound Bone Marrow In vivo medicine Animals Cytochalasin Genetics (clinical) Micronucleus Tests Demecolcine Molecular biology In vitro medicine.anatomical_structure chemistry Immunology Micronucleus test Female Bone marrow Micronucleus |
| Zdroj: | Environmental and Molecular Mutagenesis. 17:79-83 |
| ISSN: | 1098-2280 0893-6692 |
| DOI: | 10.1002/em.2850170203 |
| Popis: | The mouse micronucleus test is a valuable tool for evaluating in vivo chromosome damage produced by test articles in polychromatic erythrocytes of bone marrow. Compounds that are clastogens, such as cyclophosphamide, induce micronuclei that are smaller than those induced by compounds that are spindle poisons, such as demecolcine. In vitro studies have previously shown that the frequency of mitomycin C- and vincristine-induced micronuclei in mouse L-929 cells was reduced due to micronuclear extrusion following treatment with cytochalasin B. The current study shows that micronuclei are also expelled in vivo, that expulsion is dependent upon micronuclear size, and that observation of these extruded micronuclei is dependent upon the method of sample preparation. |
| Databáze: | OpenAIRE |
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