Malaria exposure drives both cognate and bystander human B cells to adopt an atypical phenotype
Autor: | Jedida Mwacharo, Ian A. Cockburn, Juliana Wambua, Henry J. Sutton, Francis M. Ndungu, Philip Bejon, Racheal Aye, Eunice Nduati, Jennifer N. Musyoki, Oscar Kai, Edward Otieno |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Plasmodium Immunology Plasmodium falciparum malaria Antigens Protozoan Biology Article immunological memory 03 medical and health sciences Negative selection 0302 clinical medicine Antigen parasitic diseases medicine Bystander effect Tetanus Toxoid Immunology and Allergy Humans B-cell memory B-Lymphocytes Transmission (medicine) Tetanus Toxoid Bystander Effect medicine.disease biology.organism_classification 3. Good health 030104 developmental biology Phenotype Immunologic Memory Malaria 030215 immunology |
Zdroj: | European journal of immunology |
Popis: | Atypical memory B cells (aMBCs) are found in elevated numbers in individuals exposed to malaria. A key question is whether malaria induces aMBCs as a result of exposure to Ag, or non-Ag-specific mechanisms. We identified Plasmodium and bystander tetanus toxoid (TT) specific B cells in individuals from areas of previous and persistent exposure to malaria using tetramers. Malaria-specific B cells were more likely to be aMBCs than TT-specific B cells. However, TT-specific B cells from individuals with continuous exposure to malaria were more likely to be aMBCs than TT-specific B cells in individuals from areas where transmission has ceased. Finally, sequences of BCRs specific for a blood stage malaria-Ag were more highly mutated than sequences from TT-specific BCRs and under strong negative selection, indicative of ongoing antigenic pressure. Our data suggest both persistent Ag exposure and the inflammatory environment shape the B-cell response to malaria and bystander Ags. |
Databáze: | OpenAIRE |
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