Chondrogenesis and cell proliferation in limb bud cell cultures treated with cytosine arabinoside and vitamin A
| Autor: | John R. Hassell, John P. Pennypacker, Cindy Arey Lewis |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Dose-Response Relationship
Drug Cell growth Mesenchyme Cell Cytarabine Contact inhibition Cell Count Cell Differentiation Embryo DNA Cell Biology Biology Chondrogenesis Cell biology Limb bud Cartilage medicine.anatomical_structure Biochemistry Cell culture medicine Proteoglycans Vitamin A Cell Division Cells Cultured |
| Zdroj: | Experimental Cell Research. 112:409-417 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(78)90223-9 |
| Popis: | Mesenchyme cells derived from limb buds of day 10 mouse embryos were plated out at confluent and sub-confluent cell densities. Cells in confluent cultures multiplied and differentiated into chondrocytes. The addition of vitamin A to the culture medium inhibited both cell proliferation and chondrogenesis. However, cytosine arabinoside, which also inhibited growth, did not block chondrogenesis. This indicates that the inhibition of growth in the vitamin A-treated cultures did not necessarily contribute to the inhibition of chondrogenesis. Cells in sub-confluent cultures multiplied but did not differentiate into chondrocytes. In contrast to confluent cultures, vitamin A did not inhibit growth in sub-confluent cultures. This observation suggests that vitamin A may inhibit growth by causing contact inhibition. |
| Databáze: | OpenAIRE |
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