Human milk from previously covid-19-infected mothers: The effect of pasteurization on specific antibodies and neutralization capacity
| Autor: | van Keulen, Britt J, Romijn, Michelle, Bondt, Albert, Dingess, Kelly A, Kontopodi, Eva, van der Straten, Karlijn, den Boer, Maurits A, Burger, Judith A, Poniman, Meliawati, Bosch, Berend J, Brouwer, Philip J M, de Groot, Christianne J M, Hoek, Max, Li, Wentao, Pajkrt, Dasja, Sanders, Rogier W, Schoonderwoerd, Anne, Tamara, Sem, Timmermans, Rian A H, Vidarsson, Gestur, Stittelaar, Koert J, Rispens, Theo T, Hettinga, Kasper A, van Gils, Marit J, Heck, Albert J R, van Goudoever, Johannes B, Sub Biomol.Mass Spectrometry & Proteom., Afd Biomol.Mass Spect. and Proteomics, Virologie, dI&I I&I-1, Biomolecular Mass Spectrometry and Proteomics |
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| Přispěvatelé: | Graduate School, Medical Microbiology and Infection Prevention, AII - Infectious diseases, Paediatric Infectious Diseases / Rheumatology / Immunology, ARD - Amsterdam Reproduction and Development, Landsteiner Laboratory, Neonatology, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Pediatric surgery, Obstetrics and gynaecology, Amsterdam Reproduction & Development (AR&D), AGEM - Endocrinology, metabolism and nutrition, ACS - Diabetes & metabolism, Sub Biomol.Mass Spectrometry & Proteom., Afd Biomol.Mass Spect. and Proteomics, Virologie, dI&I I&I-1, Biomolecular Mass Spectrometry and Proteomics |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
0301 basic medicine Epidemiology Bioinformatica & Diermodellen Breastfeeding Pasteurization Immunoglobulins Biology Antibodies Viral medicine.disease_cause Article Neutralization Microbiology law.invention 03 medical and health sciences law Lactation Bio-informatics & Animal models medicine Humans TX341-641 Epidemiology Bio-informatics & Animal models Coronavirus VLAG Epidemiologie 030109 nutrition & dietetics Nutrition and Dietetics Milk Human SARS-CoV-2 Nutrition. Foods and food supply Outbreak COVID-19 Antibodies Neutralizing 030104 developmental biology medicine.anatomical_structure Food Quality and Design Immunization Epidemiologie Bioinformatica & Diermodellen biology.protein Food Technology Female Antibody Food Science |
| Zdroj: | van Keulen, B J, Romijn, M, Bondt, A, Dingess, K A, Kontopodi, E, van der Straten, K, den Boer, M A, Burger, J A, Poniman, M, Bosch, B J, Brouwer, P J M, de Groot, C J M, Hoek, M, Li, W, Pajkrt, D, Sanders, R W, Schoonderwoerd, A, Tamara, S, Timmermans, R A H, Vidarsson, G, Stittelaar, K J, Rispens, T T, Hettinga, K A, van Gils, M J, Heck, A J R & van Goudoever, J B 2021, ' Human milk from previously covid-19-infected mothers: The effect of pasteurization on specific antibodies and neutralization capacity ', Nutrients, vol. 13, no. 5, 1645 . https://doi.org/10.3390/nu13051645 Nutrients, 13(5) Nutrients, Vol 13, Iss 1645, p 1645 (2021) Nutrients, 13(5):1645. Multidisciplinary Digital Publishing Institute (MDPI) Nutrients Volume 13 Issue 5 Nutrients, 13(5), 1. MDPI AG Nutrients 13 (2021) 5 |
| ISSN: | 2072-6643 |
| DOI: | 10.3390/nu13051645 |
| Popis: | Background: Since the outbreak of coronavirus disease 2019 (COVID-19), many put their hopes in the rapid availability of effective immunizations. Human milk, containing antibodies against syndrome coronavirus 2 (SARS-CoV-2), may serve as means of protection through passive immunization. We aimed to determine the presence and pseudovirus neutralization capacity of SARS-CoV-2 specific IgA in human milk of mothers who recovered from COVID-19, and the effect of pasteurization on these antibodies. Methods: This prospective case control study included lactating mothers, recovered from (suspected) COVID-19 and healthy controls. Human milk and serum samples were collected. To assess the presence of SARS-CoV-2 antibodies we used multiple complementary assays, namely ELISA with the SARS-CoV-2 spike protein (specific for IgA and IgG), receptor binding domain (RBD) and nucleocapsid (N) protein for IgG in serum, and bridging ELISA with the SARS-CoV-2 RBD and N protein for specific Ig (IgG, IgM and IgA in human milk and serum). To assess the effect of pasteurization, human milk was exposed to Holder (HoP) and High Pressure Pasteurization (HPP). Results: Human milk contained abundant SARS-CoV-2 antibodies in 83% of the proven cases and in 67% of the suspected cases. Unpasteurized milk with and without these antibodies was found to be capable of neutralizing a pseudovirus of SARS-CoV-2 in (97% and 85% of the samples respectively). After pasteurization, total IgA antibody levels were affected by HoP, while SARS-CoV-2 specific antibody levels were affected by HPP. Pseudovirus neutralizing capacity of the human milk samples was only retained with the HPP approach. No correlation was observed between milk antibody levels and neutralization capacity. Conclusions: Human milk from recovered COVID-19-infected mothers contains SARS-CoV-2 specific antibodies which maintained neutralization capacity after HPP. All together this may represent a safe and effective immunization strategy after HPP. |
| Databáze: | OpenAIRE |
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