Development of Gelatin-Coated Microspheres for Novel Bioink Design
| Autor: | Anna DeBruine, Muskan Kanungo, Yale Wang, Yuelin Wu, Emma Kroll, Lixia Ren, Noah Hutchinson, Xiaolin Hua, Wujie Zhang, Subha Kumpaty |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Scaffold
food.ingredient Polymers and Plastics Pectin Bioactive molecules Organic chemistry engineering.material scaffold Gelatin Article Microsphere gelatin food QD241-441 Coating Tissue engineering vascularization pectin Chemistry General Chemistry bioink Controlled release microspheres engineering hydrogel electrospray Biomedical engineering |
| Zdroj: | Polymers, Vol 13, Iss 3339, p 3339 (2021) Polymers Volume 13 Issue 19 |
| ISSN: | 2073-4360 |
| Popis: | A major challenge in tissue engineering is the formation of vasculature in tissue and organs. Recent studies have shown that positively charged microspheres promote vascularization, while also supporting the controlled release of bioactive molecules. This study investigated the development of gelatin-coated pectin microspheres for incorporation into a novel bioink. Electrospray was used to produce the microspheres. The process was optimized using Design-Expert® software. Microspheres underwent gelatin coating and EDC catalysis modifications. The results showed that the concentration of pectin solution impacted roundness and uniformity primarily, while flow rate affected size most significantly. The optimal gelatin concentration for microsphere coating was determined to be 0.75%, and gelatin coating led to a positively charged surface. When incorporated into bioink, the microspheres did not significantly alter viscosity, and they distributed evenly in bioink. These microspheres show great promise for incorporation into bioink for tissue engineering applications. |
| Databáze: | OpenAIRE |
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