Amyloid-β plaque deposition measured using propagation-based X-ray phase contrast CT imaging
| Autor: | Alberto Astolfo, Aurélien Lathuilière, Bernard L. Schneider, Marco Stampanoni, Vanessa Laversenne |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Nuclear and High Energy Physics
Materials science Amyloid beta amyloid plaques Phase contrast microscopy Nanotechnology Amyloid plaques Brain imaging brain imaging phase contrast 030218 nuclear medicine & medical imaging law.invention 03 medical and health sciences Mice 0302 clinical medicine Nuclear magnetic resonance Neuroimaging law Phase contrast Alzheimer Disease medicine Animals Instrumentation X-ray CT Alzheimer's disease Radiation Amyloid beta-Peptides biology medicine.diagnostic_test X-Rays X-ray Brain Magnetic resonance imaging medicine.disease Research Papers Peptide Fragments Biochemistry of Alzheimer's disease Positron emission tomography biology.protein Tomography X-Ray Computed Alzheimer’s disease 030217 neurology & neurosurgery |
| Zdroj: | Journal of Synchrotron Radiation Journal of Synchrotron Radiation, 23 (3) Journal of synchrotron radiation, Vol. 23, No Pt 3 (2016) pp. 813-819 |
| ISSN: | 1600-5775 0909-0495 |
| Popis: | Amyloid beta accumulation into insoluble plaques (Aβp) is known to play a significant role in the pathological process in Alzheimer's disease (AD). The presence of Aβp is also one of the neuropathological hallmarks for the disease. AD final diagnosis is generally acknowledged after the evaluation of Aβp deposition in the brain. Insoluble Aβp accumulation may also concur to cause AD as postulated in the so-called amyloid hypothesis. Therefore, the visualization, evaluation and quantification of Aβp are nowadays the keys for a better understanding of the disease, which may point to a possible cure for AD in the near future. Synchrotron-based X-ray phase contrast (XPC) has been demonstrated as the only imaging method that can retrieve the Aβp signal with high spatial resolution (up to 10 µm), high sensitivity and three-dimensional information at the same time. Although at the moment XPC is suitable for ex vivo samples only, it may develop into an alternative to positron emission tomography and magnetic resonance imaging in Aβp imaging. In this contribution the possibility of using synchrotron-based X-ray phase propagation computed tomography to visualize and measure Aβp on mouse brains is presented. A careful setup optimization for this application leads to a significant improvement of spatial resolution (∼1 µm), data acquisition speed (five times faster), X-ray dose (five times lower) and setup complexity, without a substantial loss in sensitivity when compared with the classic implementation of grating-based X-ray interferometry. Journal of Synchrotron Radiation, 23 (3) ISSN:0909-0495 ISSN:1600-5775 |
| Databáze: | OpenAIRE |
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