The relationship between oxygen consumption rate and viability of in vivo-derived pig embryos vitrified by the micro volume air cooling method
| Autor: | Chie Suzuki, Kiyoshi Akiyama, Koji Yoshioka, Hiroyasu Misawa, Nobutada Sakagami, Hiroyoshi Hoshi, Koji Misumi, Yuri Hirayama, K. Nishida, Shoko Yamashita |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
animal structures Pregnancy Rate Swine medicine.medical_treatment chemistry.chemical_element Biology Positive correlation Oxygen Cryopreservation Embryo Culture Techniques Andrology 03 medical and health sciences Oxygen Consumption 0302 clinical medicine Endocrinology Food Animals Pregnancy In vivo medicine Animals 030219 obstetrics & reproductive medicine Artificial insemination 0402 animal and dairy science Embryo 04 agricultural and veterinary sciences General Medicine Embryo Transfer Vitrification 040201 dairy & animal science Surgery chemistry Volume (thermodynamics) embryonic structures Tissue and Organ Harvesting Female Animal Science and Zoology |
| Zdroj: | Animal Reproduction Science. 164:40-46 |
| ISSN: | 0378-4320 |
| DOI: | 10.1016/j.anireprosci.2015.11.008 |
| Popis: | The aim of this study was to assess the viability of vitrified-warmed in vivo-derived pig embryos after measuring the oxygen consumption rate. Six days after artificial insemination, blastocysts were collected from gilts and vitrified by the micro volume air cooling method. The oxygen consumption rate was measured in 60 vitrified-warmed embryos, which were then cultured for 48h to assess the viability. The survival (re-expansion) rate of embryos after warming was 85.0%. The average oxygen consumption rate of embryos immediately after warming was greater in embryos which could re-expand during subsequent culture (F=0.75±0.04) than that in those which failed to re-expand (F=0.33±0.05). Moreover, the oxygen consumption rate of vitrified-warmed embryos was greater in the hatched (F=0.88±0.06) than that in the not-hatched group (F=0.53±0.04). When the oxygen consumption rate of the vitrified-warmed embryos and the numbers of viable and dead cells in embryos were determined, there was a positive correlation between the oxygen consumption rate and the number of live cells (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect