Susceptibility to biofilm formation on 3D-printed titanium fixation plates used in the mandible: a preliminary study
| Autor: | Katarzyna Baldy-Chudzik, Justyna Mazurek-Popczyk, Lukasz Palka, Katarzyna Arkusz |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 3d printed Materials science chemistry.chemical_element Infectious and parasitic diseases RC109-216 Microbiology bacterial contamination 03 medical and health sciences Fixation (surgical) 0302 clinical medicine Dentistry (miscellaneous) Fixation plate Orthodontics Mandible Biofilm 3D printing 030206 dentistry surface topography QR1-502 030104 developmental biology Infectious Diseases chemistry biofilm formation Oral and maxillofacial surgery Original Article Research Article Titanium |
| Zdroj: | Journal of Oral Microbiology article-version (VoR) Version of Record Journal of Oral Microbiology, Vol 12, Iss 1 (2020) |
| ISSN: | 2000-2297 |
| DOI: | 10.1080/20002297.2020.1838164 |
| Popis: | Background: In the oral and maxillofacial surgery, fixation plates are commonly used for the stabilization of bone fragments. Additive manufacturing has enabled us to design and create personalized fixation devices that would ideally fit any given fracture. Aim: The aim of the present preliminary study was to assess the susceptibility of 3D-printed titanium fixation plates to biofilm formation. Methods: Plates were manufactured using selective laser melting (SLM) from Ti-6Al-4 V. Reference strains of Streptococcus mutans, Staphyloccocus epidermidis, Staphylococcus aureus, Lactobacillus rhamnosus, and Candida albicans, were tested to evaluate the material’s susceptibility to biofilm formation over 48 hours. Biofilm formations were quantified by a colorimetric method and colony-forming units (CFU) quantification. Scanning electron microscopy (SEM) visualized the structure of the biofilm. Results: Surface analysis revealed the average roughness of 102.75 nm and irregular topography of the tested plates. They were susceptible to biofilm formation by all tested strains. The average CFUs were as follows: S. mutans (11.91 x 107) > S.epidermidis (4.45 x 107) > S. aureus (2.3 x 107) > C.albicans (1.22 x 107) > L. rhamnosus (0.78 x 107). Conclusions: The present preliminary study showed that rough surfaces of additively manufactured titanium plates are susceptible to microbial adhesion. The research should be continued in order to compare additively manufactured plates with other commercially available osteotomy plates. Therefore, we suggest caution when using this type of material. |
| Databáze: | OpenAIRE |
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