Mechanical difference between white and gray matter in the rat cerebellum measured by scanning force microscopy
| Autor: | Hélène O. B. Gautier, Andreas F. Christ, Pouria Moshayedi, James W. Fawcett, Kristian Franze, Jochen Guck, Ragnhildur Thóra Káradóttir, Robin J.M. Franklin |
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| Rok vydání: | 2010 |
| Předmět: |
Materials science
Models Neurological Biomedical Engineering Biophysics Grey matter Microscopy Atomic Force White matter Rats Sprague-Dawley Optics Indentation Cerebellum Elastic Modulus Microscopy medicine Animals Orthopedics and Sports Medicine Elasticity (economics) Elastic modulus business.industry Rehabilitation Biomechanics Stiffness Biomechanical Phenomena Rats medicine.anatomical_structure Female Stress Mechanical medicine.symptom business Biomedical engineering |
| Zdroj: | Journal of biomechanics. 43(15) |
| ISSN: | 1873-2380 |
| Popis: | The mechanical properties of tissues are increasingly recognized as important cues for cell physiology and pathology. Nevertheless, there is a sparsity of quantitative, high-resolution data on mechanical properties of specific tissues. This is especially true for the central nervous system (CNS), which poses particular difficulties in terms of preparation and measurement. We have prepared thin slices of brain tissue suited for indentation measurements on the micrometer scale in a near-native state. Using a scanning force microscope with a spherical indenter of radius ∼20μm we have mapped the effective elastic modulus of rat cerebellum with a spatial resolution of 100μm. We found significant differences between white and gray matter, having effective elastic moduli of K=294±74 and 454±53Pa, respectively, at 3μm indentation depth (n(g)=245, n(w)=150 in four animals, p |
| Databáze: | OpenAIRE |
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