Investigation of Chlamydiaceae in semen and cauda epididymidis and seroprevalence of Chlamydophila abortus in breeding bulls
| Autor: | Ylva Persson, Ann-Charlotte Karlsson, Stefan Alenius, Camilla Björkman, Stina Englund |
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| Jazyk: | angličtina |
| Předmět: |
DNA
Bacterial Male Veterinary medicine endocrine system animal diseases Cattle Diseases Semen Beef cattle Breeding law.invention Andrology fluids and secretions law Seroepidemiologic Studies Seroprevalence Animals Chlamydiaceae Animal Husbandry Chlamydophila Infections Polymerase chain reaction Epididymis Chlamydophila lcsh:Veterinary medicine General Veterinary biology urogenital system Research General Medicine biology.organism_classification Antibodies Bacterial Chlamydophila abortus Fertility lcsh:SF600-1100 Cattle Female |
| Zdroj: | Acta Veterinaria Scandinavica Acta Veterinaria Scandinavica, Vol 52, Iss 1, p 2 (2010) |
| ISSN: | 1751-0147 |
| DOI: | 10.1186/1751-0147-52-2 |
| Popis: | Background Reproductive disorders associated with chlamydial infection have been reported worldwide in cattle and there are indications of potential venereal transmission. Methods Semen samples from 21 dairy bulls and cauda epididymidis tissue samples from 43 beef bulls were analysed for chlamydial agent by real-time polymerase chain reaction (PCR) including an internal amplification control (mimic). Additionally, presence of antibodies against Chlamydophila (Cp.) abortus among the bulls was investigated with the commercial Pourquier® ELISA Cp. abortus serum verification kit. Results No chlamydial agent was detected by PCR in either the semen samples or in the tissue samples. Additionally, no antibodies against Cp. abortus were detected. Conclusions The results suggest that Cp. abortus is very rare, or absent in Swedish bulls and thus the risk for venereal transmission of chlamydial infection through their semen is low. However, because Chlamydophila spp. infection rates seem to differ throughout the world, it is essential to clarify the relative importance of transmission of the infection through semen on cattle fertility. |
| Databáze: | OpenAIRE |
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