Interaction of G protein Gbetagamma dimers with small GTP-binding proteins of the Rho family
| Autor: | Rainer Harhammer, Gtinter Schultz, Antje Gohla |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Small GTPase
G protein Biophysics GTPgammaS Plasma protein binding Biology Biochemistry chemistry.chemical_compound GTP-binding protein regulators Biopolymers Structural Biology Rho GTP-Binding Proteins Heterotrimeric G protein Genetics Animals Molecular Biology fungi Cell Biology Fusion protein Guanine Nucleotides Cell biology Rac G beta-gamma complex chemistry Gβγ dimer Cattle Transducin Protein Binding Signal Transduction |
| Zdroj: | FEBS letters. 399(3) |
| ISSN: | 0014-5793 |
| Popis: | Gbetagamma dimers of heterotrimeric G proteins have been shown to be important for the translocation of cytosolic proteins to membranes. The involvement of Gbetagamma in those signaling processes mediated by small GTP-binding proteins of the Rho family was studied using purified proteins. We showed specific binding of bovine brain Gbetagamma to immobilized GST-Rho fusion proteins. In addition, brain Gbetagamma, but not transducin Gbetagamma, was able to inhibit GTPgammaS binding to GST-Rho in a concentration-dependent manner. GTPgammaS binding to GST-Rac was also decreased by brain Gbetagamma whereas nucleotide binding to GST-Cdc42 was not changed. We conclude that Gbetagamma dimers may participate in the process of membrane attachment and/or other regulations of Rho and Rac. |
| Databáze: | OpenAIRE |
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