Photodynamic Therapy Enhanced the Antitumor Effects of Berberine on HeLa Cells
| Autor: | Li Minghua, Hu Azhen, Guang-Hui Cui, Han-Qing Liu, Ya-Wen An |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
biology medicine.medical_treatment hela cell Photodynamic therapy General Chemistry biology.organism_classification HeLa 03 medical and health sciences chemistry.chemical_compound Chemistry 0302 clinical medicine Berberine chemistry photodynamic therapy 030220 oncology & carcinogenesis berberine mtor Materials Chemistry Cancer research medicine QD1-999 PI3K/AKT/mTOR pathway 030304 developmental biology |
| Zdroj: | Open Chemistry, Vol 17, Iss 1, Pp 413-421 (2019) |
| ISSN: | 2391-5420 |
| Popis: | In this study we investigated the antineoplastic effects of Berberine (BBR)-mediated photodynamic therapy (PDT) on HeLa cells and its related mechanisms. The CCK-8 assay and flow cytometry were used to evaluate the proliferation and apoptosis of cells respectively. In addition, changes in protein expression levels were assessed using western blot. BBR at dose of 10 mg/kg was injected intraperitoneally to mice with tumors and PDT treatments were performed 24 hours later. In vivo imaging systems were used to evaluate the fluorescence of BBR. In vitro, PDT significantly enhanced the effects of BBR on inducing cell apoptosis and inhibiting proliferation. The in vivo results showed that the fluorescence intensity in the PDT group was decreased compared with that in the BBR group. Tumor weights and tumor size in the PDT group were less than those in the control group; however, when BBR was applied without PDT, no significant differences were observed between the BBR and control group. The results of western blot showed that PDT enhanced the inhibitory effects of BBR on the mammalian target of rapamycin (mTOR) signaling pathway, that may partly explain the potential underlying mechanisms. |
| Databáze: | OpenAIRE |
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