Light-Controlled Chemoenzymatic Immobilization of Proteins towards Engineering of Bioactive Papers
| Autor: | Olga Avrutina, Tobias Meckel, Markus Biesalski, Aileen Ebenig, Harald Kolmar, Desislava Yanakieva, Valentina Hilberg |
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| Rok vydání: | 2019 |
| Předmět: |
Paper
Light Point-of-Care Systems 010402 general chemistry 01 natural sciences Catalysis Enzyme catalysis Bacterial Proteins Sortase Fluorescent Dyes Microscopy Confocal Cycloaddition Reaction 010405 organic chemistry Protein immobilization Chemistry Organic Chemistry Stereoisomerism General Chemistry Aminoacyltransferases Amides Combinatorial chemistry 0104 chemical sciences Cysteine Endopeptidases Immobilized Proteins Covalent bond Sortase A Biocatalysis Bioactive paper Peptides Biosensor |
| Zdroj: | Chemistry - A European Journal. 25:1746-1751 |
| ISSN: | 0947-6539 |
| DOI: | 10.1002/chem.201804889 |
| Popis: | Efficient and reliable methods for the generation of bioactive papers are of growing interest in relation to point-of-care testing devices that do not require extensive analytical equipment. Herein, we report the immobilization of functional proteins on paper fibers using a modular chemoenzymatic approach. The synthetic strategy relies on a combination of highly efficient spatially controllable photo-triggered cycloaddition followed by site-specific sortase A-catalyzed transamidation. This site-directed and regiospecific method has allowed unidirectional and covalent immobilization of several proteins displaying different functional properties, with ramifications for application in paper-based diagnostics. |
| Databáze: | OpenAIRE |
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