Application of immunomagnetic particles to enzyme-linked immunosorbent assay (ELISA) for improvement of detection sensitivity of HCG
| Autor: | Jay Z. Yeh, Chii Ming Jiang, Po Hua Wu, Ming Chang Wu, Hsiao Ting Kuo |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Pregnancy test
endocrine system Horse radish peroxidase medicine.drug_class Clinical Biochemistry Immunology Enzyme-Linked Immunosorbent Assay Double antibody sandwich Monoclonal antibody Chorionic Gonadotropin Sensitivity and Specificity Antibodies Human chorionic gonadotropin Gestational period medicine Immunology and Allergy Humans Magnetite Nanoparticles reproductive and urinary physiology chemistry.chemical_classification Chromatography biology urogenital system Chemistry Immunomagnetic Separation Molecular biology Medical Laboratory Technology Enzyme Cross-Linking Reagents biology.protein Antibody hormones hormone substitutes and hormone antagonists |
| Zdroj: | Journal of immunoassayimmunochemistry. 33(4) |
| ISSN: | 1532-4230 |
| Popis: | This investigation was aimed at using superparamagnetic particles to enzyme-linked immunosorbent assay (SPIO-ELISA) of human chorionic gonadotropin (hCG) to enhance detection sensitivity of hCG. We found that N-(3-dimethyl aminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) was the best cross-linking reagent to link anti hCG α antibody to superparamagnetic particle (SPIO-anti hCG α antibody immunomagnetic particle). To improve the specificity of the assay, a horse radish peroxidase (HRP)-labeled anti-hCG beta monoclonal antibody was used to detect captured hCG using double antibody sandwich ELISA assay. SPIO-ELISA application to determine hCG increased the sensitivity to 1 mIU/mL, which is a level of sensitivity enabling the diagnosis of pregnancy during the early gestational period. |
| Databáze: | OpenAIRE |
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