The NTP generating activity of pyruvate kinase II is critical for apicoplast maintenance in Plasmodium falciparum
| Autor: | Cyrianne Keutcha, Amanda Dziedzic, Sean T. Prigge, Hans B. Liu, Bobby Kwan, Krithika Rajaram, Russell P. Swift, Anne E. Jedlicka |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
carbon metabolism
QH301-705.5 Science Pyruvate Kinase malaria Chemical biology Biology General Biochemistry Genetics and Molecular Biology Organelle Nucleotide Biology (General) Gene chemistry.chemical_classification Apicoplast apicoplast General Immunology and Microbiology General Neuroscience Plasmodium falciparum General Medicine biology.organism_classification Cell biology Enzyme plasmodium chemistry Medicine microarray Pyruvate kinase |
| Zdroj: | eLife, Vol 9 (2020) |
| ISSN: | 2050-084X |
| DOI: | 10.7554/elife.50807 |
| Popis: | The apicoplast of Plasmodium falciparum parasites is believed to rely on the import of three-carbon phosphate compounds for use in organelle anabolic pathways, in addition to the generation of energy and reducing power within the organelle. We generated a series of genetic deletions in an apicoplast metabolic bypass line to determine which genes involved in apicoplast carbon metabolism are required for blood-stage parasite survival and organelle maintenance. We found that pyruvate kinase II (PyrKII) is essential for organelle maintenance, but that production of pyruvate by PyrKII is not responsible for this phenomenon. Enzymatic characterization of PyrKII revealed activity against all NDPs and dNDPs tested, suggesting that it may be capable of generating a broad range of nucleotide triphosphates. Conditional mislocalization of PyrKII resulted in decreased transcript levels within the apicoplast that preceded organelle disruption, suggesting that PyrKII is required for organelle maintenance due to its role in nucleotide triphosphate generation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|