Neonatal pertussis presenting as acute bronchiolitis: direct detection of the Bordetella pertussis genome using loop-mediated isothermal amplification
| Autor: | Ryuzo Hanayama, Akari Nakamura, Hiroko Shimoda, Yoshihisa Kinoshita, Katsuhiro Nomoto, Toshiyuki Ohta, Takashi Sakano, Takeki Furue, Yasuyuki Okada, Tetsuo Nakayama, Hiroaki Ono, Tetsushi Suto |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
DNA
Bacterial Bordetella pertussis Lymphocytosis Whooping Cough Loop-mediated isothermal amplification Infant Newborn Diseases Diagnosis Differential Clarithromycin medicine Humans Piperacillin biology business.industry Apneic episodes Infant Newborn medicine.disease Early infancy biology.organism_classification Anti-Bacterial Agents Acute Bronchiolitis Bronchiolitis Acute Disease Pediatrics Perinatology and Child Health Immunology B. pertussis Female medicine.symptom business Nucleic Acid Amplification Techniques |
| Zdroj: | European Journal of Pediatrics. 168:347-349 |
| ISSN: | 1432-1076 0340-6199 |
| DOI: | 10.1007/s00431-008-0744-8 |
| Popis: | We report a 28-day-old female infant with pertussis presenting as severe acute bronchiolitis with cyanosis. On admission, the patient's symptoms were similar to those of acute bronchiolitis. However, occasional apneic episodes with cyanosis and peripheral lymphocytosis suggested neonatal pertussis and prompted us to examine the presence of Bordetella pertussis using loop-mediated isothermal amplification (LAMP) based on the insertion sequence IS481. LAMP of the nasopharyngeal and intratracheal aspirates was positive for B. pertussis and a diagnosis of neonatal pertussis was made. As the clinical features of pertussis in neonates and early infancy are not characteristic, LAMP is a useful tool for rapid diagnosis of B. pertussis infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink