Development of a panel of recombinase polymerase amplification assays for detection of common bacterial urinary tract infection pathogens
| Autor: | Sri Rajagopalan, Richard C. Willson, Archana Marapadaga, Katerina Kourentzi, Balakrishnan Raja, Heather J. Goux |
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| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Klebsiella pneumoniae Urinary system 030106 microbiology Recombinase Polymerase Amplification DNA-Directed DNA Polymerase Biology medicine.disease_cause Polymerase Chain Reaction Applied Microbiology and Biotechnology Article Enterococcus faecalis Microbiology Recombinases 03 medical and health sciences medicine Humans Escherichia coli Retrospective Studies Bacteria Pseudomonas aeruginosa Bacterial Infections General Medicine biology.organism_classification Virology Proteus mirabilis genomic DNA Urinary Tract Infections Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 123:544-555 |
| ISSN: | 1365-2672 1364-5072 |
| Popis: | To develop and evaluate the performance of a panel of isothermal real-time recombinase polymerase amplification (RPA) assays for detection of common bacterial urinary tract infection (UTI) pathogens.The panel included RPAs for Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa and Enterococcus faecalis. All five RPAs required reaction times of under 12 min to reach their lower limit of detection of 100 genomes per reaction or less, and did not cross-react with high concentrations of nontarget bacterial genomic DNA. In a 50-sample retrospective clinical study, the five-RPA assay panel was found to have a specificity of 100% (95% CI, 78-100%) and a sensitivity of 89% (95% CI, 75-96%) for UTI detection.The analytical and clinical validity of RPA for the rapid and sensitive detection of common UTI pathogens was established.Rapid identification of the causative pathogens of UTIs can be valuable in preventing serious complications by helping avoid the empirical treatment necessitated by traditional urine culture's 48-72-h turnaround time. The routine and widespread use of RPA to supplement or replace culture-based methods could profoundly impact UTI management and the emergence of multidrug-resistant pathogens. |
| Databáze: | OpenAIRE |
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