Enhanced and Differential Capture of Circulating Tumor Cells from Lung Cancer Patients by Microfluidic Assays Using Aptamer Cocktail
| Autor: | Li Xu, Chuanhao Tang, Hongjun Gao, Haixu Hu, Xiaoyan Li, Wei Zhou, Mengfei Huang, Bing Liu, Zhen Zhang, Yi Liu, Anna Fong, Hsian-Rong Tseng, Si Cheng, Libo Zhao, Xiaohong Fang |
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| Rok vydání: | 2016 |
| Předmět: |
Silicon
Lung Neoplasms Tumor State Aptamer Microfluidics 02 engineering and technology 010402 general chemistry Bioinformatics 01 natural sciences Tumor heterogeneity Article Fluorescence Biomaterials Circulating tumor cell Cell Line Tumor medicine Humans General Materials Science Lung cancer Nanowires business.industry Reproducibility of Results General Chemistry Oncology problem Aptamers Nucleotide Neoplastic Cells Circulating 021001 nanoscience & nanotechnology medicine.disease 0104 chemical sciences Cancer research Non small cell 0210 nano-technology business Biotechnology |
| Zdroj: | Small. 12:1072-1081 |
| ISSN: | 1613-6810 |
| DOI: | 10.1002/smll.201503188 |
| Popis: | Collecting circulating tumor cells (CTCs) shed from solid tumor through a minimally invasive approach provides an opportunity to solve a long-standing oncology problem, the real-time monitoring of tumor state and analysis of tumor heterogeneity. However, efficient capture and detection of CTCs with diverse phenotypes is still challenging. In this work, a microfluidic assay is developed using the rationally-designed aptamer cocktails with synergistic effect. Enhanced and differential capture of CTCs for nonsmall cell lung cancer (NSCLC) patients is achieved. It is also demonstrated that the overall consideration of CTC counts obtained by multiple aptamer combinations can provide more comprehensive information in treatment monitoring. |
| Databáze: | OpenAIRE |
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