Modified expression of ZmMYB167 in Brachypodium distachyon and Zea mays leads to increased cell wall lignin and phenolic content
| Autor: | Rosario Iacono, Maurice Bosch, Ondrej Kosik, Joe Gallagher, Odin M. Moron-Garcia, Susan Dalton, Rakesh Bhatia, Luned Roberts |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Propanols Lignocellulosic biomass Biomass lcsh:Medicine Molecular engineering in plants complex mixtures Lignin Zea mays Article Ferulic acid 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Phenols Gene Expression Regulation Plant Botany MYB Amino Acid Sequence lcsh:Science Plant Proteins 2. Zero hunger Multidisciplinary biology Cell wall lcsh:R fungi Transgenic plants food and beverages biology.organism_classification Plants Genetically Modified 030104 developmental biology Phenotype chemistry Solubility Brachypodium lcsh:Q Brachypodium distachyon Sugars Secondary cell wall 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2045-2322 |
| Popis: | One of the challenges to enable targeted modification of lignocellulosic biomass from grasses for improved biofuel and biochemical production lies within our limited understanding of the transcriptional control of secondary cell wall biosynthesis. Here, we investigated the role of the maize MYB transcription factor ZmMYB167 in secondary cell wall biosynthesis and how modified ZmMYB167 expression in two distinct grass model species affects plant biomass and growth phenotypes. Heterologous expression of ZmMYB167 in the C3 model system Brachypodium led to mild dwarf phenotypes, increased lignin (~7% to 13%) and S-lignin monomer (~11% to 16%) content, elevated concentrations of cell wall-bound p-coumaric acid (~15% to 24%) and reduced biomass sugar release (~20%) compared to controls. Overexpression of ZmMYB167 in the C4 model system Zea mays increased lignin (~4% to 13%), p-coumaric acid (~8% to 52%) and ferulic acid (~13% to 38%) content but did not affect plant growth and development nor biomass recalcitrance. Taken together, modifying ZmMYB167 expression represents a target to alter lignin and phenolic content in grasses. The ZmMYB167 expression-induced discrepancies in plant phenotypic and biomass properties between the two grass model systems highlight the challenges and opportunities for MYB transcription factor-based genetic engineering approaches of grass biomass. |
| Databáze: | OpenAIRE |
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