Prefluorescent Nitroxide Probe for the Highly Sensitive Determination of Peroxyl and Other Radical Oxidants
| Autor: | Yu Tang, Sarah A. Green, Yiu-Fai Lam, Neil V. Blough, Min Jia |
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| Rok vydání: | 2009 |
| Předmět: |
Detection limit
Nitroxide mediated radical polymerization Chromatography Chemistry Fluorescence spectrometry Oxidants Fluorescamine Photochemistry Fluorescence High-performance liquid chromatography Fluorescence spectroscopy Peroxides Analytical Chemistry Cyclic N-Oxides chemistry.chemical_compound Liposomes Indicators and Reagents Derivatization Chromatography High Pressure Liquid |
| Zdroj: | Analytical Chemistry. 81:8033-8040 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac901374m |
| Popis: | Fluorescamine derivatized 3-amino-2,2,5,5,-tetramethyl-1-pyrrolidinyloxy (I) is shown to undergo an irreversible reaction with peroxyl radicals and other radical oxidants to generate a more highly fluorescent diamagnetic product (II) and thus can be used as a highly sensitive and versatile probe to determine oxidant production optically, either by monitoring the changes in fluorescence intensity, by HPLC analysis with fluorescence detection, or by a combination of both approaches. By changing the [O2]/[I] ratio, we show that peroxyl radicals can be detected and quantified preferentially in the presence of other radical oxidants. Detection of photochemically produced peroxyl radicals is achieved by employing 3-amino-2,2,5,5,-tetramethyl-1-pyrrolidinyloxy (3-ap) alone, followed by derivatization with fluorescamine. With employment of HPLC analysis, the detection limit of II at a S/N of 2 is approximately 3 nM for a 125 microL injection. Preliminary applications include the detection of peroxyl radicals generated thermally in soybean phosphatidylcholine liposomes and produced photochemically in tap water. |
| Databáze: | OpenAIRE |
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