Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells
| Autor: | Pavel Skalický, Martina Karasová, Josef Slavík, Miroslav Ciganek, Jiří Ehrmann, Petra Ovesná, Alois Kozubík, Ondřej Zapletal, Jan Vondráček, Jiřina Procházková, Jan Bouchal, Zlatka Hušková, Zdeněk Kolář, Jiřina Hofmanová, Zuzana Tylichová, Miroslav Machala, Monika Levková, Nicol Straková |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Fatty Acid Desaturases
Male chemistry.chemical_compound 0302 clinical medicine Biology (General) Spectroscopy chemistry.chemical_classification 0303 health sciences biology Fatty Acids desaturation General Medicine 3. Good health Computer Science Applications Gene Expression Regulation Neoplastic Chemistry Isolated Tumor Cells Fatty acid synthase Lysophosphatidylcholine 030220 oncology & carcinogenesis Colonic Neoplasms Female Stearoyl-CoA Desaturase Fatty Acid Elongases QH301-705.5 Adenocarcinoma Article Catalysis Inorganic Chemistry 03 medical and health sciences colorectal carcinoma Humans Physical and Theoretical Chemistry QD1-999 Molecular Biology Fatty acid synthesis phospholipids Aged 030304 developmental biology lysophospholipids fatty acid synthesis Fatty acid metabolism Lipogenesis Organic Chemistry Lysophosphatidylethanolamine Fatty acid Lipid Metabolism Molecular biology epithelial cells Fatty acid desaturase chemistry EpCAM biology.protein lipidomics Fatty Acid Synthases |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 13 International Journal of Molecular Sciences, Vol 22, Iss 6650, p 6650 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22136650 |
| Popis: | The development of colon cancer, one of the most common malignancies, is accompanied with numerous lipid alterations. However, analyses of whole tumor samples may not always provide an accurate description of specific changes occurring directly in tumor epithelial cells. Here, we analyzed in detail the phospholipid (PL), lysophospholipid (lysoPL), and fatty acid (FA) profiles of purified EpCAM+ cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients. We found that a number of FAs increased significantly in isolated tumor cells, which also included a number of long polyunsaturated FAs. Higher levels of FAs were associated with increased expression of FA synthesis genes, as well as with altered expression of enzymes involved in FA elongation and desaturation, including particularly fatty acid synthase, stearoyl-CoA desaturase, fatty acid desaturase 2 and ELOVL5 fatty acid elongase 5 We identified significant changes in ratios of specific lysoPLs and corresponding PLs. A number of lysophosphatidylcholine and lysophosphatidylethanolamine species, containing long-chain and very-long chain FAs, often with high numbers of double bonds, were significantly upregulated in tumor cells. Increased de novo synthesis of very long-chain FAs, or, altered uptake or incorporation of these FAs into specific lysoPLs in tumor cells, may thus contribute to reprogramming of cellular phospholipidome and membrane alterations observed in colon cancer. |
| Databáze: | OpenAIRE |
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