Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia
| Autor: | Gleb Haynatzki, George P. Casale, Matthew Fuglestad, Julian K S Kim, Holly DeSpiegelaere, Gregory Willcockson, Constance J. Mietus, Petros Karvelis, Panagiotis Koutakis, Christina Shields, Hernan Hernandez, Nicholas Lambert, Timothy J Lackner, Iraklis I. Pipinos |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty αSMA+ pericytes basement membrane thickening lcsh:Medicine 030204 cardiovascular system & hematology Article Masson's trichrome stain 03 medical and health sciences 0302 clinical medicine Fibrosis medicine Myocyte microvascular pathology Pathological 030304 developmental biology Basement membrane 0303 health sciences business.industry fibrosis lcsh:R General Medicine Critical limb ischemia medicine.disease medicine.anatomical_structure Pericyte medicine.symptom Claudication business |
| Zdroj: | Journal of Clinical Medicine, Vol 9, Iss 2575, p 2575 (2020) Journal of Clinical Medicine Volume 9 Issue 8 |
| ISSN: | 2077-0383 |
| Popis: | Work from our laboratory documents pathological events, including myofiber oxidative damage and degeneration, myofibrosis, micro-vessel (diameter = 50&ndash 150 &mu m) remodeling, and collagenous investment of terminal micro-vessels (diameter &le 15 µ m) in the calf muscle of patients with Peripheral Artery Disease (PAD). In this study, we evaluate the hypothesis that the vascular pathology associated with the legs of PAD patients encompasses pathologic changes to the smallest micro-vessels in calf muscle. Biopsies were collected from the calf muscle of control subjects and patients with Fontaine Stage II and Stage IV PAD. Slide specimens were evaluated by Quantitative Multi-Spectral and Fluorescence Microscopy. Inter-myofiber collagen, stained with Masson Trichrome (MT), was increased in Stage II patients, and more substantially in Stage IV patients in association with collagenous thickening of terminal micro-vessel walls. Evaluation of the Basement Membrane (BM) of these vessels reveals increased thickness in Stage II patients, and increased thickness, diameter, and Collagen I deposition in Stage IV patients. Coverage of these micro-vessels with pericytes, key contributors to fibrosis and BM remodeling, was increased in Stage II patients, and was greatest in Stage IV patients. Vascular pathology of the legs of PAD patients extends beyond atherosclerotic main inflow arteries and affects the entire vascular tree&mdash including the smallest micro-vessels. |
| Databáze: | OpenAIRE |
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