Immunization of Teenagers with a Fifth Dose of Reduced DTaP-IPV Induces High Levels of Pertussis Antibodies with a Significant Increase in Opsonophagocytic Activity
| Autor: | Audun Aase, Synne Sandbu, Tove Karin Herstad, Merete Bolstad, Samuel Merino, Ingeborg S. Aaberge, Hilde Bakke |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Microbiology (medical) Bordetella pertussis Adolescent Whooping Cough Clinical Biochemistry Immunology Immunization Secondary Enzyme-Linked Immunosorbent Assay Booster dose Diphtheria-Tetanus-acellular Pertussis Vaccines Pertussis toxin Immunoglobulin G Microbiology Phagocytosis medicine Humans Immunology and Allergy Virulence Factors Bordetella Adhesins Bacterial Whooping cough biology Opsonin Proteins biology.organism_classification medicine.disease Antibodies Bacterial Immunoglobulin A Poliovirus Vaccine Inactivated Pertussis Toxin IgG binding biology.protein Female Microbial Immunology Antitoxins Pertactin Bacterial Outer Membrane Proteins |
| Zdroj: | Clinical and Vaccine Immunology. 18:1269-1274 |
| ISSN: | 1556-679X 1556-6811 |
| DOI: | 10.1128/cvi.05067-11 |
| Popis: | Waning vaccine-induced immunity againstBordetella pertussisis observed among adolescents and adults. A high incidence of pertussis has been reported in this population, which serves as a reservoir forB. pertussis. A fifth dose of reduced antigen of diphtheria-tetanus-acellular-pertussis and inactivated polio vaccine was given as a booster dose to healthy teenagers. The antibody activity againstB. pertussisantigens was measured prior to and 4 to 8 weeks after the booster by different assays: enzyme-linked immunosorbent assays (ELISAs) of IgG and IgA against pertussis toxin (PT) and filamentous hemagglutinin (FHA), IgG against pertactin (PRN), opsonophagocytic activity (OPA), and IgG binding to liveB. pertussis. There was a significant increase in the IgG activity against PT, FHA, and PRN following the booster immunization (P< 0.001). The prebooster sera showed a geometric mean OPA titer of 65.1 and IgG binding to live bacteria at a geometric mean concentration of 164.9 arbitrary units (AU)/ml. Following the fifth dose, the OPA increased to a titer of 360.4, and the IgG concentration against live bacteria increased to 833.4 AU/ml (P< 0.001 for both). The correlation analyses between the different assays suggest that antibodies against FHA and PRN contribute the most to the OPA and IgG binding. |
| Databáze: | OpenAIRE |
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