Site-Selective Binding of Nanoparticles to Double-Stranded DNA via Peptide Nucleic Acid 'Invasion'
| Autor: | Daniel van der Lelie, Mathew M. Maye, Dazhi Sun, Oleg Gang, Andrea L. Stadler |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Peptide Nucleic Acids
Base Sequence Peptide nucleic acid General Engineering Nucleic Acid Hybridization General Physics and Astronomy DNA Biology Molecular biology Nucleic acid thermodynamics chemistry.chemical_compound Chimera (genetics) Microscopy Electron Transmission chemistry Duplex (building) Colloidal gold Nucleic acid Biophysics Nanoparticles Spectrophotometry Ultraviolet General Materials Science Sequence motif DNA Primers |
| Zdroj: | ACS Nano. 5:2467-2474 |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/nn101355n |
| Popis: | We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)-DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands. At the same time, the single-stranded (ss) DNA tail of the chimera, allows for anchoring of nano-objects that have been functionalized with complementary ssDNA. The developed method is applied for interparticle attachment and for the fabrication of particle clusters using a dsDNA template. This method significantly broadens the molecular toolbox for constructing nanoscale systems by including the most conventional not yet utilized DNA motif, double helix DNA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|