Molar concentration from sequential 2-D water-window X-ray ptychography and X-ray fluorescence in hydrated cells
| Autor: | Mark D. Junker, M. D. de Jonge, G. A. van Riessen, Kirstin Elgass, Michael W. M. Jones |
|---|---|
| Rok vydání: | 2023 |
| Předmět: |
0301 basic medicine
Molar concentration Materials science Analytical chemistry X-ray fluorescence 01 natural sciences Article 03 medical and health sciences Mice X-Ray Diffraction 0103 physical sciences Microscopy Fluorescence microscope Image Processing Computer-Assisted Animals 010306 general physics Cells Cultured Uncategorized Water window Multidisciplinary X-ray Spectrometry X-Ray Emission Fibroblasts Elements Fluorescence Ptychography Extracellular Matrix 030104 developmental biology |
| Zdroj: | Scientific Reports |
| DOI: | 10.26181/22114559.v1 |
| Popis: | Recent developments in biological X-ray microscopy have allowed structural information and elemental distribution to be simultaneously obtained by combining X-ray ptychography and X-ray fluorescence microscopy. Experimentally, these methods can be performed simultaneously; however, the optimal conditions for each measurement may not be compatible. Here, we combine two distinct measurements of ultrastructure and elemental distribution, with each measurement performed under optimised conditions. By combining optimised ptychography and fluorescence information we are able to determine molar concentrations from two-dimensional images, allowing an investigation into the interactions between the environment sensing filopodia in fibroblasts and extracellular calcium. Furthermore, the biological ptychography results we present illustrate a point of maturity where the technique can be applied to solve significant problems in structural biology. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|