Expression profiling of metalloproteinases and their inhibitors in cartilage
| Autor: | Andrew E. Parker, Dylan R. Edwards, Simon T. Donell, Lara Kevorkian, Sarah Porter, Ian M. Clark, David Young, Lee Shepstone, C. Darrah, S.M. Brockbank |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male MMP3 Pathology medicine.medical_specialty MMP1 Disintegrins Immunology Biology Osteoarthritis Hip Fractures Bone Rheumatology ADAMTS1 Protein Gene expression medicine Humans Immunology and Allergy Pharmacology (medical) Femur Aged TIMP1 Aged 80 and over Regulation of gene expression Gene Expression Profiling Cartilage ADAMTS Metalloendopeptidases Tissue Inhibitor of Metalloproteinases Middle Aged Molecular biology Gene expression profiling ADAM Proteins medicine.anatomical_structure Metalloproteases Female |
| Zdroj: | Arthritis & Rheumatism. 50:131-141 |
| ISSN: | 1529-0131 0004-3591 |
| DOI: | 10.1002/art.11433 |
| Popis: | Objective To profile the expression of all known members of the matrix metalloproteinase (MMP), ADAMTS, and tissue inhibitor of metalloproteinases (TIMP) gene families in normal cartilage and cartilage from patients with osteoarthritis (OA). Methods Human cartilage was obtained from femoral heads at joint replacement for OA or following fracture to the femoral neck. Total RNA was purified, and gene expression was assayed using quantitative real-time polymerase chain reaction. Results Several members of the above gene families were regulated in OA. Genes that showed increased expression in OA were MMP13, MMP28, and ADAMTS16 (all at P < 0.001), MMP9, MMP16, ADAMTS2, and ADAMTS14 (all at P < 0.01), and MMP2, TIMP3, and ADAMTS12 (all at P < 0.05). Genes with decreased expression in OA were MMP1, MMP3, and ADAMTS1 (all at P < 0.001), MMP10, TIMP1, and ADAMTS9 (all at P < 0.01), and TIMP4, ADAMTS5, and ADAMTS15 (all at P < 0.05). Correlation analysis revealed that groups of genes across the gene families were coexpressed in cartilage. Conclusion This is the first comprehensive expression profile of all known MMP, ADAMTS, and TIMP genes in cartilage. Elucidation of patterns of expression provides a foundation with which to understand mechanisms of gene regulation in OA and potentially to refine the specificity of antiproteolytic therapies. |
| Databáze: | OpenAIRE |
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