Iron upregulates melanogenesis in cultured retinal pigment epithelial cells
| Autor: | Yafeng Li, Arvydas Maminishkis, Jared Iacovelli, Joshua L. Dunaief, Oleg Alekseev, Natalie Wolkow, Ying Song, Jennifer C. Lee, Delu Song |
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| Rok vydání: | 2014 |
| Předmět: |
Tyrosinase
Blotting Western Gestational Age Retinal Pigment Epithelium Real-Time Polymerase Chain Reaction Ferric Compounds Article Cellular and Molecular Neuroscience chemistry.chemical_compound Downregulation and upregulation medicine Humans RNA Messenger Transcription factor Cells Cultured Melanosome Melanins Genetics Melanosomes Membrane Glycoproteins Retinal pigment epithelium Monophenol Monooxygenase Intracellular Signaling Peptides and Proteins Retinal Microphthalmia-associated transcription factor Tissue Donors Sensory Systems Up-Regulation Cell biology Intramolecular Oxidoreductases Quaternary Ammonium Compounds Ophthalmology medicine.anatomical_structure chemistry sense organs Carrier Proteins Oxidoreductases Dopachrome tautomerase gp100 Melanoma Antigen |
| Zdroj: | Experimental Eye Research. 128:92-101 |
| ISSN: | 0014-4835 |
| Popis: | The purpose of our studies was to examine the relationship between iron and melanogenesis in retinal pigment epithelial cells, as prior observations had suggested that iron may promote melanogenesis. This relationship has potential clinical importance, as both iron overload and hyperpigmentation are associated with age-related macular degeneration (AMD). Human fetal retinal pigment epithelial cells and ARPE-19 cells were treated with iron in the form of ferric ammonium citrate, after which quantitative RT-PCR and electron microscopy were performed. Melanogenesis genes tyrosinase, tyrosinase-related protein 1, Hermansky-Pudlak Syndrome 3, premelanosome protein and dopachrome tautomerase were upregulated, as was the melanogenesis-controlling transcription factor, microphthalmia-associated transcription factor (MITF). Iron-treated cells had increased pigmentation and melanosome number. Multiple transcription factors upstream of MITF were upregulated by iron. |
| Databáze: | OpenAIRE |
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