A single step duplex PCR to distinguishEntamoeba histolyticafromEntamoeba dispar
| Autor: | Marco Gomes, Almir S. Martins, Michelle A. R. Freitas, E. N. Vianna, Jorge L. Pesquero, Edward F. Silva |
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| Rok vydání: | 2004 |
| Předmět: |
Dispar
Oligonucleotide Primer Biology Lobosea Polymerase Chain Reaction law.invention Feces chemistry.chemical_compound Entamoeba histolytica fluids and secretions Species Specificity law parasitic diseases Animals Humans Gene Polymerase chain reaction DNA Protozoan biology.organism_classification Virology Molecular biology Actins Cysteine Endopeptidases Infectious Diseases chemistry Dysentery Amebic Protozoa Animal Science and Zoology Parasitology DNA |
| Zdroj: | Parasitology. 128:625-628 |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182004005086 |
| Popis: | In this study, a single-step duplex polymerase chain reaction procedure was developed for rapid, specific and sensitive identification ofEntamoeba histolyticaand for its diagnostic differentiation fromE. dispar. Specific oligonucleotide primers were combined for the amplification of a cysteine proteinase 5 gene target sequence of 242 bp, present only inE. histolytica. Additionally, another oligonucleotide primer pair for both theE. histolyticaandE. disparactin gene target of 300 bp was designed to amplify only from amoebae DNA. The PCR developed was specific and efficiently identified and differentiated these parasites from each other in either cultured parasites or from stool material. |
| Databáze: | OpenAIRE |
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