Predicting Confined 1D Cell Migration from Parameters Calibrated to a 2D Motor-Clutch Model
| Autor: | David J. Odde, Louis S. Prahl, Maria R. Stanslaski, Matthieu Piel, Pablo Vargas |
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| Přispěvatelé: | University of Minnesota [Twin Cities] (UMN), University of Minnesota System, Biologie Cellulaire et Cancer, Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Immunité et cancer (U932), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Curie [Paris] |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Microfluidics Cell Biophysics [SDV.BC]Life Sciences [q-bio]/Cellular Biology Extracellular matrix 03 medical and health sciences 0302 clinical medicine Cell Movement medicine Humans Clutch Cytoskeleton 030304 developmental biology 0303 health sciences Cell migration Articles Glioma Extracellular Matrix Membrane medicine.anatomical_structure Self-healing hydrogels 030217 neurology & neurosurgery |
| Zdroj: | Biophysical Journal Biophysical Journal, Biophysical Society, 2020, 118 (7), pp.1709-1720. ⟨10.1016/j.bpj.2020.01.048⟩ |
| ISSN: | 0006-3495 1542-0086 |
| DOI: | 10.1016/j.bpj.2020.01.048⟩ |
| Popis: | International audience; Biological tissues contain micrometer-scale gaps and pores, including those found within extracellular matrix fiber networks, between tightly packed cells, and between blood vessels or nerve bundles and their associated basement membranes. These spaces restrict cell motion to a single-spatial dimension (1D), a feature that is not captured in traditional in vitro cell migration assays performed on flat, unconfined two-dimensional (2D) substrates. Mechanical confinement can variably influence cell migration behaviors, and it is presently unclear whether the mechanisms used for migration in 2D unconfined environments are relevant in 1D confined environments. Here, we assessed whether a cell migration simulator and associated parameters previously measured for cells on 2D unconfined compliant hydrogels could predict 1D confined cell migration in microfluidic channels. We manufactured microfluidic devices with narrow channels (60-μm2 rectangular cross-sectional area) and tracked human glioma cells that spontaneously migrated within channels. Cell velocities (vexp = 0.51 ± 0.02 μm min−1) were comparable to brain tumor expansion rates measured in the clinic. Using motor-clutch model parameters estimated from cells on unconfined 2D planar hydrogel substrates, simulations predicted similar migration velocities (vsim = 0.37 ± 0.04 μm min−1) and also predicted the effects of drugs targeting the motor-clutch system or cytoskeletal assembly. These results are consistent with glioma cells utilizing a motor-clutch system to migrate in confined environments. |
| Databáze: | OpenAIRE |
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